This article describes microinjection and electroporation of mouse testis invivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.
Michaelis, M., Sobczak, A., Weitzel, J. M. In Vivo Microinjection and Electroporation of Mouse Testis. J. Vis. Exp. (90), e51802, doi:10.3791/51802 (2014).