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In Vivo Microinjection and Electroporation of Mouse Testis
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo Microinjection and Electroporation of Mouse Testis

In Vivo Microinjection and Electroporation of Mouse Testis

27,125 Views

08:39 min

August 23, 2014

DOI:

08:39 min
August 23, 2014

28 Views
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Summary

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This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

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