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In Vivo Microinjection and Electroporation of Mouse Testis
 

In Vivo Microinjection and Electroporation of Mouse Testis

Article DOI: 10.3791/51802 08:39 min
August 23rd, 2014

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Summary August 23rd, 2014

This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

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