Musculoskeletal Tissue Analysis
Musculoskeletal Tissue Analysis - Related Videos
07:49
Isolating Stem Cells from Soft Musculoskeletal Tissues
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Cited by 21 •
2010
Isolating adult stem cells from musculoskeletal soft tissues based on the cell's adherence speed to flask.
10:53
A Novel Application of Musculoskeletal Ultrasound Imaging
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Cited by 14 •
2013
We describe a new ultrasound-based vector tissue Doppler imaging technique to measure muscle contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities. This approach provides complementary measurements of dynamic muscle function and could lead to a better understanding of mechanisms underlying musculoskeletal disorders.
09:53
Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
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Cited by 1 •
2025
This method describes the exploitation of the endogenous signal in an ARL13B-CENTRIN-2 transgenic mouse model to image primary cilia in situ, in three dimensions across large areas of mineralized tissue, from tissue collection to analysis of cilia organization. This protocol may also be applied to other types of tissues.
08:52
3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue
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Cited by 34 •
2017
3D ultrasound imaging (3DUS) allows fast and cost-effective morphometry of musculoskeletal tissues. We present a protocol to measure muscle volume and fascicle length using 3DUS.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
FAQs
No single measurement captures tissue behavior completely. Histology and microscopy reveal cellular organization and extracellular matrix, imaging characterizes structural features, biochemical assays assess composition such as mineral content, and mechanical testing measures load-bearing behavior. Combining these approaches allows researchers to relate microscopic changes and material composition to functional differences in bone, cartilage, muscle, and connective tissues.
The relevant features depend on the tissue and research question, but analyses may examine cellular organization, extracellular matrix, mineral content, structural appearance, and mechanical performance. Considering several features together helps distinguish changes in composition from changes in organization or function. This integrated perspective is especially useful when characterizing health, disease, injury, or abnormal development.
Comparison provides a reference for identifying biologically meaningful changes. Differences between normal and altered samples can indicate tissue degeneration, injury, inflammation, or abnormal development, depending on the measured structural, compositional, or mechanical feature. Using matched analytical categories across samples also helps connect observed tissue abnormalities with changes in function or disease-related processes.
Microscopy can show how cells and extracellular matrix are organized, but it does not directly describe how a tissue responds to load. Mechanical testing supplies information about load-bearing behavior, allowing structural or compositional observations to be considered alongside function. This connection is important for evaluating musculoskeletal alterations and for studying tissues whose performance depends on both organization and material properties.
A study generally begins by selecting normal or altered tissue samples and defining the structural, compositional, or functional features of interest. Researchers then apply appropriate combinations of histology, microscopy, imaging, biochemical assays, and mechanical testing. Results are compared across samples to characterize tissue condition and relate cellular organization, matrix, mineral content, and load-bearing behavior.
This approach supports investigations of aging, disease mechanisms, biomaterials, rehabilitation, and regenerative medicine. Researchers can use structural and compositional measurements to examine degeneration, injury, inflammation, or abnormal development, while mechanical measurements provide functional context. Together, these outcomes help evaluate altered tissues and inform efforts to understand, repair, replace, or restore musculoskeletal function.