Methylene Chloride
Methylene Chloride - Related Videos
06:05
Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection
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Cited by 1 •
2017
Methylene blue dye injection into the renal pelvis facilitates the assessment of urinary tract junction obstruction defects during mouse embryonic urinary tract development. Here, a protocol for methylene blue dye injection into the renal pelvis is described.
07:32
Dynamic Electrochemical Measurement of Chloride Ions
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Cited by 5 •
2016
Dynamic measurement of chloride ions is presented. Transition time of an Ag/AgCl electrode, during a chronopotentiometric technique, can give the concentration of chloride ions in electrolyte. This method does not require a stable conventional reference electrode.
10:37
Ferric Chloride-induced Murine Thrombosis Models
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Cited by 56 •
2016
We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation.
04:33
Methylene Blue Staining to Assess Metastatic Colony Formation In Vitro: A Staining Procedure to Assess Metastasis by Quantifying Harvested Cancer Cells From Cancer Mouse Model
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2025
In this video, we describe the methylene blue staining procedure to quantify the metastatic burden by counting stained colonies obtained by culturing cancer cells harvested from distant organs of a mouse model following injection.
03:47
Methylene Blue Dye Injection in Mouse Embryonic Urinary Tract: A Method To Assess the Congenital Obstruction in the Urinary Tract
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2025
In this video, we administer methylene blue dye injection into the urinary tract of a perinatal embryonic mouse to assess congenital anomalies related to urinary tract obstruction.
FAQs
Partitioning reflects the solvent’s moderate polarity and its ability to dissolve many organic substances. When an aqueous sample contains compounds with greater affinity for the organic solvent, those compounds can move into the methylene chloride phase, separating them from water-soluble material. This behavior supports preparation of organic-phase samples for later analysis.
Moderate polarity helps methylene chloride dissolve a broad range of organic compounds, while its low boiling point allows the solvent to evaporate readily after extraction. Together, these properties support recovery of an organic fraction without retaining the solvent indefinitely. The same volatility also makes vapor control and appropriate laboratory ventilation essential during use.
Methylene chloride can serve during preparation of samples intended for chromatography by helping transfer selected organic compounds into an organic phase. Removing the solvent afterward produces a prepared fraction for chromatographic analysis. In biological research, this approach is relevant when investigators need to examine extracted lipids, metabolites, or other organic substances rather than the original aqueous mixture.
A general workflow begins with an aqueous sample and introduces methylene chloride so compounds can partition between aqueous and organic phases. The organic fraction is then separated for analysis or further processing, and the solvent may be evaporated because of its low boiling point. The exact handling sequence depends on the sample and the intended analytical application.
Use requires appropriate ventilation, protective equipment, and careful solvent handling because methylene chloride is volatile and its vapors can affect the nervous system. Prolonged exposure presents additional health risks, so limiting vapor accumulation is an important part of experimental practice. These precautions apply during extraction, chromatography preparation, evaporation, and other open handling steps.
Biological applications include lipid extraction, metabolite extraction, chromatography, and preparation of organic-phase samples for analysis. The method is most useful when the investigation requires separation of organic substances from an aqueous sample. The resulting fraction can help researchers examine selected chemical components instead of analyzing the starting biological material as a single, unseparated mixture.