Myogenic Endothelial Cells
Myogenic Endothelial Cells - Related Videos
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
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Cited by 32 •
2014
In vitro culture systems have proven indispensible to our understanding of vertebrate myogenesis. However, much remains to be learned about nonmammalian skeletal muscle development and growth, particularly in basal taxa. An efficient and robust protocol for isolating the adult stem cells of this tissue, the myogenic precursor cells (MPCs), and maintaining their self-renewal, proliferation, and differentiation in a primary culture setting allows for the identification of conserved and divergent...
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
10:03
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
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Cited by 30 •
2014
Induced pluripotent stem cell (iPSC)-derived myogenic progenitors are promising candidates for cell therapy strategies to treat muscular dystrophies. This protocol describes transplantation and functional measurements required to evaluate the engraftment and differentiation of iPSC-derived mesoangioblasts (a type of muscle progenitors) in mouse models of acute and chronic muscle regeneration.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
FAQs
Developmental or regenerative conditions can activate muscle-specific gene programs in these endothelial-lineage cells. Local signals from nearby blood vessels, muscle fibers, and supporting cells help shape this response, influencing whether the cells change phenotype and acquire myoblast-like or muscle-forming characteristics. This context dependence is important when interpreting their behavior in normal development, tissue repair, or experimental systems.
Some cells can activate muscle-related programs and differentiate toward myoblast-like or muscle-forming cells without completely losing endothelial features. This combination indicates that their phenotype may remain flexible rather than switching irreversibly from one identity to another. Recognizing this mixed state helps researchers examine how vascular and muscle properties coexist during regeneration and how cell identity changes under different local conditions.
Angiogenesis, the formation or growth of blood-vessel networks, and myogenesis, the development of muscle, are closely connected through shared local environments. Myogenic endothelial cells provide a way to study how vascular cells and muscle-forming processes influence one another. Understanding this relationship may clarify how coordinated tissue organization supports skeletal muscle formation and repair.
Studies focus on settings in which endothelial-lineage cells encounter signals associated with skeletal muscle formation or repair. Developmental contexts reveal how muscle-specific programs can emerge, whereas regenerative contexts show how phenotype changes may contribute to tissue restoration. Comparing these conditions helps identify how blood vessels, muscle fibers, and supporting cells shape the cells’ behavior.
Their dual relationship with vascular and muscle biology makes them relevant to vascularized tissue engineering, which aims to consider blood-vessel integration alongside muscle formation. Research on these cells may help explain how vascular and muscle components can be studied together rather than as isolated systems. The broader goal is to inform strategies for constructing or repairing tissues with coordinated functions.
In muscle injury research, investigators can examine whether local regenerative signals influence endothelial-lineage cells to activate muscle programs or adopt myoblast-like features. These observations may inform cell-based therapy research by identifying how vascular context affects repair-related behavior. The cells therefore connect questions about tissue regeneration, endothelial plasticity, and the conditions needed for effective muscle restoration.