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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
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Bioengineering
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JoVE Journal Bioengineering
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

DOI:
10.3791/50532-v

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10:03 min

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January 20, 2014

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, , ,
1Department of Cell and Developmental Biology,University College London, 2Division of Regenerative Medicine, Stem Cells and Gene Therapy,San Raffaele Hospital

Chapters

  • 00:05Title
  • 01:48Assessment of Myogenic Potential
  • 02:53Transplantation in Mouse Models of Muscular Dystrophy
  • 06:37Outcome Measures on Transplanted Dystrophic Animals: Treadmill Test
  • 08:15Results: In vitro and In vivo Differentiation and Motor Capacity after Transplantation
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

Induced pluripotent stem cell (iPSC)-derived myogenic progenitors are promising candidates for cell therapy strategies to treat muscular dystrophies. This protocol describes transplantation and functional measurements required to evaluate the engraftment and differentiation of iPSC-derived mesoangioblasts (a type of muscle progenitors) in mouse models of acute and chronic muscle regeneration.

Tags

Keywords: Induced Pluripotent Stem CellsMesoangioblast-like Myogenic ProgenitorsMuscular DystrophyGenetic CorrectionMyoD OverexpressionIn Vitro DifferentiationIn Vivo TransplantationAcute And Chronic Muscle RegenerationFunctional TestsCell EngraftmentDifferentiation

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