N. Benthamiana Plants
N. Benthamiana Plants - Related Videos
08:14
Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
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Cited by 3 •
2013
A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.
07:33
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
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Cited by 61 •
2014
Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
11:09
An Easy Method for Plant Polysome Profiling
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Cited by 20 •
2016
This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.
02:12
Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing
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2026
Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...
FAQs
Agrobacterium delivers a foreign genetic construct into cells within the leaf, where the introduced information can direct production of the target protein or other experimental product. Because expression occurs without establishing a stable transgenic line, researchers can examine the construct over a short experimental period and rapidly assess its biological activity or cellular location.
The leaves readily support agroinfiltration, making them practical sites for introducing genetic constructs into plant cells. Combined with the species' fast growth, this feature shortens the interval between construct delivery and experimental observation. Researchers can therefore evaluate gene function, protein behavior, or pathogen-related processes more efficiently than approaches requiring prolonged development of stable plant lines.
Transient expression provides a short-term test of a foreign construct without requiring researchers to generate and maintain a stable transgenic line. This distinction makes the system valuable for initial hypothesis testing and rapid construct assessment. Stable lines may support longer-term studies, whereas the transient approach emphasizes speed and experimental flexibility when results are needed within days.
Protein expression in these leaves can support several kinds of analysis, including examining protein production, determining subcellular localization, and testing biological function. Localization studies reveal where a protein accumulates within plant cells, while functional tests can connect a genetic construct with a cellular or physiological effect. These complementary outcomes help researchers evaluate molecular hypotheses quickly.
Researchers can introduce genetic constructs into N. benthamiana leaf cells to examine how particular genes or expressed proteins relate to plant-pathogen interactions. The rapid, transient format allows candidate factors or responses to be tested without first producing stable transgenic plants. This makes the system useful for connecting molecular changes with processes relevant to infection and plant defense.
N. benthamiana provides a plant context in which virus-induced gene silencing can be investigated alongside other gene-function approaches. By reducing the activity of selected genes, researchers can examine resulting changes and infer those genes' contributions to plant biology. Its rapid experimental cycle supports efficient screening of hypotheses before more extended studies are undertaken.
The system combines rapid testing with the ability to produce foreign proteins in plant tissue, supporting research on plant-based vaccines, diagnostics, and other recombinant products. Researchers can first evaluate genetic constructs and expression outcomes in leaves, then use the resulting information to guide development of candidate products or production platforms.