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An Easy Method for Plant Polysome Profiling

An Easy Method for Plant Polysome Profiling

Article DOI: 10.3791/54231
August 28th, 2016


Summary August 28th, 2016

This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.


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