Nipple Injection Technique
Nipple Injection Technique - Related Videos
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
07:34
Minimally-invasive Technique for Injection into Rat Optic Nerve
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Cited by 4 •
2015
Direct injection into the rat optic nerve is useful for regenerative research. We demonstrate a minimally-invasive technique for direct injection into a rat optic nerve that does not involve opening the skull. Using this method, surgical complications are minimized and recovery is more rapid.
06:39
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
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Cited by 36 •
2013
A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.
09:08
Facilitating Repeat Intracarotid Injections in Mouse Models by a Novel Injection Site Repair Technique
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Cited by 1 •
2024
Repair of the intracarotid artery in a mouse model after injection returns blood flow to the artery without negatively impacting the distribution of the injected material. Injection site repair facilitates subsequent injections through the same artery and prevents cerebral ischemia in mouse strains that lack a complete Circle of Willis.
03:40
Orthotopic Injection into the Mammary Fat Pad: Establishing Breast Cancer in Mice
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2023
In this article we discuss how to establish a breast cancer model in female mice by injecting breast cancer cells into the mammary fat pad. The example protocol demonstrates the orthotopic injection.
FAQs
Entry through the nipple opening places the solution at the beginning of the mammary ductal system, allowing delivery along a defined internal pathway. Distribution therefore occurs within the ducts rather than beginning broadly in surrounding tissue. This anatomical route helps investigators examine localized effects on mammary epithelial compartments and tissue organization.
Controlled delivery helps regulate how much solution enters the ductal system and supports more consistent exposure within the targeted mammary compartment. By limiting unnecessary spread into nearby tissues, this approach can improve experimental precision. Consistent placement and delivery are particularly relevant when comparing mammary development, epithelial responses, or reactions to candidate treatments.
The ductal system provides the anatomical context in which delivered substances contact mammary epithelial tissue. Effects observed after administration can therefore be related to duct-associated organization and function rather than treated as responses from the entire surrounding region. This makes the method useful for examining how mammary structures respond during development, tumor formation, or treatment studies.
The procedure requires a delivery device capable of entering the nipple opening, typically a fine needle or cannula, together with the experimental solution. The device supports controlled placement into the ductal system, while the solution carries the substance under investigation. These components provide the basic means to target a defined mammary compartment in biological experiments.
A researcher introduces a fine needle or cannula through the nipple opening and delivers the experimental solution in a controlled manner. The intended result is distribution through the mammary ducts while exposure of surrounding tissues remains limited. Subsequent analysis can focus on changes in mammary tissue organization, epithelial biology, development, or treatment response.
This approach is relevant when an experiment requires direct access to the mammary ductal compartment rather than broad exposure of nearby tissues. It can support studies of mammary gland development, epithelial biology, tumor formation, and responses to candidate treatments. The localized route is especially useful when anatomical precision is important for interpreting tissue-level outcomes.