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Minimally-invasive Technique for Injection into Rat Optic Nerve
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Minimally-invasive Technique for Injection into Rat Optic Nerve
DOI:

07:34 min

May 19, 2015

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Chapters

  • 00:05Title
  • 01:27Anesthesia and Positioning
  • 02:46Dissection
  • 04:05Pipette Injector and Injection
  • 05:43Follow Up
  • 06:29Results: Gross Dissection of Injected Optic Nerve
  • 07:07Conclusion

Summary

Automatic Translation

Direct injection into the rat optic nerve is useful for regenerative research. We demonstrate a minimally-invasive technique for direct injection into a rat optic nerve that does not involve opening the skull. Using this method, surgical complications are minimized and recovery is more rapid.

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