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Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
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Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

DOI:
10.3791/50692-v

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06:39 min

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October 04, 2013

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, ,
1Vascular Biology Program, Department of Surgery,Boston Children’s Hospital and Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering,Harvard University, 3Harvard School of Engineering and Applied Sciences

Chapters

  • 00:05Title
  • 01:16Pre-operative Preparation
  • 02:48Intraductal Injection and Analysis of Mammary Gland Tissue
  • 05:02Results: Mammary Gland Injections Enable Localized Delivery of Molecules
  • 06:15Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Tags

Keywords: Intraductal InjectionLocalized Drug DeliveryMouse Mammary GlandEvans BlueFluorescent ReagentsNon-invasiveSiRNAChemotherapeutic AgentsSmall Molecules

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