Non-coding Rna
Non-coding Rna - Related Videos
10:39
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
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Cited by 40 •
2016
This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Cited by 59 •
2018
This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.
09:40
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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2018
Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood sample.
02:39
lncRNA - Long Non-coding RNAs
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2020
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
05:27
RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells
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Cited by 4 •
2023
The present protocol describes a method of RNA fluorescence in situ hybridization to localize the lncRNAs in human osteosarcoma cells.
FAQs
MicroRNAs pair with target messenger RNAs to influence what happens to those transcripts. This pairing can promote messenger RNA degradation or inhibit translation, reducing the protein-producing output from a targeted gene. The mechanism makes sequence-specific RNA regulation relevant when researchers examine how gene expression changes across biological conditions.
Long non-coding RNAs can regulate genes by recruiting proteins, guiding regulatory complexes, or altering chromatin states. These mechanisms allow them to influence the regulatory environment around genetic information rather than acting only through direct effects on messenger RNA. Their varied activities help explain how cells control gene expression and maintain distinct cellular programs.
The classes differ according to the cellular processes they support. MicroRNAs regulate messenger RNAs, whereas long non-coding RNAs can organize regulatory activities through protein recruitment, complex guidance, or chromatin changes. Ribosomal, transfer, and small nuclear RNAs support core operations including translation and RNA splicing, showing that non-coding RNAs contribute to both regulation and basic cell function.
Non-coding RNA provides mechanisms for controlling gene expression and cellular regulation, processes that are central to development and cell identity. Differences in these regulatory activities can help explain how cells acquire or maintain distinct characteristics. Studying these RNAs therefore connects molecular regulation with broader biological questions about how cells develop and function.
Because non-coding RNAs regulate gene expression, genome organization, and cellular function, changes involving them can offer clues about disease mechanisms. Their study may help researchers connect altered molecular regulation with abnormal cellular behavior. This makes non-coding RNA a useful subject for investigating how disease-related processes arise and identifying biological features worth further study.
Research on non-coding RNA can support biomarker discovery by identifying RNA-related features associated with biological or disease states. The same regulatory mechanisms also motivate potential RNA-based therapeutic strategies, particularly when controlling gene expression could be useful. These applications extend the topic from basic biology toward methods for detecting or influencing cellular processes.