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JoVE Journal
Genetics

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
 

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Article DOI: 10.3791/57379
April 10th, 2018

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Summary April 10th, 2018

This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.

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