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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
JoVE Journal
Genetics
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JoVE Journal Genetics
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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09:36 min

April 10, 2018

DOI:

09:36 min
April 10, 2018

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Summary

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This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.

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