On-chip Pump
On-chip Pump - Related Videos
01:30
ATP Driven Pumps III: V-type Pumps
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2023
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
11:16
A Microfluidic Chip for ICPMS Sample Introduction
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Cited by 4 •
2015
We present a discrete droplet sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). It is based on a cheap and disposable microfluidic chip that generates highly monodisperse droplets in a size range of 40−60 µm at frequencies from 90 to 7,000 Hz.
14:58
Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping
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Cited by 17 •
2015
The fabrication process and experimental characterization techniques relevant to single-electron pumps based on silicon metal-oxide-semiconductor quantum dots are discussed.
01:34
ATP Driven Pumps II: P-type Pumps
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2023
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V. A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
FAQs
Device design determines how liquid is moved and controlled. Pneumatic membrane actuation can drive flow through membrane movement, while peristaltic deformation transports liquid through sequential channel deformation. Electroosmotic forces and capillary pressure provide alternative driving mechanisms. Because these mechanisms differ, researchers select a design according to whether an experiment requires transport, mixing, or recirculation within the microfluidic platform.
Flow regulation affects more than liquid delivery. An on-chip pump can establish chemical gradients and control shear stress, the conditions created by moving liquid along biological interfaces. These conditions are relevant when studying cells or tissues in controlled microenvironments. Precise regulation can also improve consistency between experiments by making media delivery and fluid exposure more reproducible.
Integration places fluid actuation within the microfluidic platform rather than depending on bulky external equipment. This arrangement can reduce the size of the experimental setup while preserving control over media delivery, gradients, and shear stress. The result is a more integrated approach that may support portable systems, automated workflows, or experiments where space and reagent use are limited.
The intended fluid operation guides pump selection and use. Transport moves liquid through miniature channels, mixing combines fluid streams, and recirculation repeatedly returns liquid within the system. Pneumatic, peristaltic, electroosmotic, and capillary-pressure designs can provide these functions through different driving mechanisms. Matching the mechanism to the required operation helps researchers regulate experimental conditions without adding unnecessary external equipment.
A typical workflow begins with integrating the pump into the microfluidic platform, then using its actuation mechanism to establish controlled liquid movement. Researchers can direct delivery, mixing, or recirculation according to the experiment while creating the desired chemical gradients and shear stress. This workflow connects fluid handling directly with biological modules such as cell cultures, organ-on-chip models, or biochemical assays.
Cell culture, organ-on-chip models, biochemical assays, and automated sample preparation are key applications. In cell-based systems, controlled media delivery and shear stress can help create defined experimental conditions. Organ-on-chip platforms can use the same fluid control within miniature biological models, while assays and sample-preparation workflows benefit from reduced reagent use and greater integration.