Oral Mucosal Organoids
Oral Mucosal Organoids - Related Videos
10:43
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
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Cited by 10 •
2022
This protocol describes the key steps to generate and characterize murine oral-esophageal 3D organoids that represent normal, preneoplastic, and squamous cell carcinoma lesions induced via chemical carcinogenesis.
04:28
Utilizing an Orally Dissolving Strip for Pharmacological and Toxicological Studies: A Simple and Humane Alternative to Oral Gavage for Animals
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Cited by 4 •
2016
The following protocol describes a novel method for chronic oral drug administration using an orally dissolving strip (ODS) in lieu of the more commonly used oral gavage method. We demonstrate that preclinical, oral drug delivery using the ODS method represents a safe, convenient, and humane alternative to oral gavage.
02:55
Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid
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2025
This video demonstrates the quantification of immune mediator levels in neonatal human nasal mucosal lining fluid extract. An electrochemiluminescence-based multiplex assay system measures these mediators by evaluating the intensity of emitted light when they are excited.
08:46
Nose, Sinuses, Oral Cavity and Pharynx Exam
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2023
Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA This video provides an overview of sinus, nose, and throat examinations. The demonstration begins with a brief overview of the anatomy of the region. The upper third of the nose is bony, and the bottom two-thirds are cartilaginous. Air entering the nares passes through the nasal vestibules and into the narrow passageway between the nasal septum...
01:25
Mucosal Barrier of the Stomach
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2024
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl. Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
FAQs
Oral epithelial stem and progenitor cells provide the renewing population needed to maintain tissue growth. Within a supportive matrix, these cells expand and organize into structures that preserve epithelial renewal while also producing differentiated cells. This combination allows the model to represent both ongoing epithelial maintenance and maturation, which are central processes in oral tissue biology.
The supportive matrix provides an environment in which oral epithelial cells can expand and self-organize, while defined culture conditions guide tissue development. Together, these factors help preserve the balance between renewal and differentiation. Controlling this environment gives researchers a more consistent system for examining how oral epithelial tissues develop and maintain their functional organization.
Oral mucosal organoids provide tissue-like organization in a controlled human-cell-based system, rather than representing cells only as a conventional culture or relying entirely on an animal model. They can therefore complement both approaches by preserving aspects of epithelial structure and function in vitro, while allowing researchers to study oral biology under experimentally controlled conditions.
Generation begins with oral epithelial stem or progenitor cells, which are expanded in a supportive matrix. Under defined culture conditions, the cells self-organize into tissue-like structures that maintain epithelial renewal and differentiation. This workflow creates a laboratory model suitable for subsequent investigation of oral tissue biology, repair, host–microbe interactions, or disease-related changes.
These organoids support studies of oral epithelial barrier function, wound repair, and interactions between host tissue and microbes. They can also be used to examine diseases affecting the mouth, linking tissue organization with biologically relevant responses. Because the system is maintained in vitro, researchers can investigate these processes in a controlled experimental setting.
Researchers can use these models to evaluate potential therapeutics and to study patient-specific responses when organoids are generated from appropriate patient-derived material. Their preserved epithelial organization makes them relevant for testing questions connected to oral disease and tissue repair. They also complement conventional cultures and animal models when human tissue behavior is important.