Pax3 Pax7
Pax3 Pax7 - Related Videos
07:18
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Cited by 23 •
2018
The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.
09:38
Direct Reprogramming of Mouse Fibroblasts into Melanocytes
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Cited by 2 •
2021
Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.
07:56
Isolation of Skeletal Muscle Satellite Cells for In Vitro Myogenesis Studies
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2026
This protocol provides a standardized method for isolating and enriching primary myoblasts from adult and neonatal mouse skeletal muscle through tissue dissociation, sequential filtration, preplating, and defined culture conditions, enabling the establishment and maintenance of satellite cell-derived myoblast cultures for downstream experimental applications.
FAQs
The paired domain is the central molecular feature that connects these factors to gene regulation. By recognizing specific DNA sequences, Pax3 and Pax7 can influence whether target genes are activated or repressed. Those transcriptional effects alter programs controlling cell fate, migration, proliferation, and differentiation, linking DNA binding to developmental and regenerative outcomes.
Although both proteins belong to the same paired box transcription-factor group, their emphasized biological roles differ. Pax3 is particularly associated with neural crest and skeletal muscle development, while Pax7 is closely linked to the maintenance of quiescent satellite cells. Comparing them therefore helps separate early tissue formation from the preservation of muscle stem-cell states.
Pax7 is important because it marks and helps maintain quiescent satellite cells. These muscle stem cells support growth and repair, so Pax7 connects a measurable molecular feature with the population responsible for those processes. Examining it can therefore clarify how muscle stem-cell maintenance relates to tissue regeneration.
During embryonic development, examining Pax3 and Pax7 connects transcriptional regulation with the formation of specialized tissues. Their target-gene effects can be considered in relation to cell fate, migration, proliferation, and differentiation, while Pax3 provides particular insight into neural crest and skeletal muscle development. This makes the pair useful for interpreting how tissues are established.
Because Pax3 and Pax7 regulate developmental gene expression, their activity can be examined in relation to congenital disorders. Pax3-centered analysis is especially relevant where neural crest or skeletal muscle development is involved, whereas Pax7-centered analysis highlights muscle stem-cell maintenance. This distinction helps connect molecular regulation with tissue-level developmental abnormalities.
Regenerative biology can use Pax3 and Pax7 as molecular entry points for studying muscle repair and stem-cell behavior. Pax7 is particularly relevant because it marks and helps maintain quiescent satellite cells, the stem-cell population that supports muscle growth and repair. Understanding these regulatory roles can inform strategies aimed at manipulating stem cells.