Photopigment Molecules

Photopigment molecules are light-absorbing compounds that convert photons into chemical changes, enabling organisms to sense light or use it as an energy source. Their chromophores absorb particular wavelengths; photon absorption can trigger molecular rearrangements, as retinal isomerization activates opsin proteins in visual systems, while chlorophyll transfers excitation energy during photosynthesis. In biology, these molecules help explain color vision, phototaxis, circadian responses, and solar-energy capture, making them central to studies of sensory physiology, plant function, and evolution. Characterizing their absorption spectra and reaction pathways also supports research on vision disorders, optogenetics, and bio-inspired light-harvesting materials.

Photopigment Molecules - Related Videos

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JoVE Journal - Neuroscience

Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors

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Cited by 2 •

2022

We present a protocol to electrophysiologically characterize bi-stable photopigments: (i) exploiting the charge displacements within the photopigment molecules following photon-absorption and their huge amount in the photoreceptors, and (ii) exploiting the absorption-spectra differences of rhodopsin and metarhodopsin photopigment states. These protocols are useful to screen for mutations affecting bi-stable photopigment...

Investigating Prolonged Depolarizing Afterpotential (PDA) in Drosophila Photoreceptors

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2025

This video demonstrates an experimental protocol to investigate the prolonged depolarizing afterpotential (PDA) in white-eyed Drosophila. The fly's eye is exposed to intense blue light pulses to convert the photopigment rhodopsin to metarhodopsin, initiating a signaling cascade that opens positive ion channels and causes membrane depolarization. Because blue light prevents the reconversion of metarhodopsin to rhodopsin, the continuous influx of positive ions results in sustained depolarization,...

Two-Photon Microscopy for Monitoring Calcium Dynamics in Mouse Retinal Cone Photoreceptors

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2025

Source: Kulkarni, M. et al. Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina. J. Vis. Exp. (2015)This video demonstrates the use of two-photon microscopy to monitor calcium dynamics in cone photoreceptors of a transgenic mouse retina. It outlines the steps for preparing retinal slices, imaging FRET-based calcium biosensors, and analyzing fluorescence changes to quantify calcium levels under background illumination and light stimulation.

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