Pinning Procedure Steps

Pinning procedure steps describe the systematic preparation of preserved insect specimens for collection, identification, and anatomical study. After an insect is positioned with its body and appendages arranged naturally, an entomological pin is inserted through the appropriate part of the thorax, and wings or legs are adjusted with mounting supports; the specimen is then allowed to dry and receives a label containing collection data. Consistent pinning preserves diagnostic structures, reduces handling damage, and creates organized reference material for taxonomy, biodiversity surveys, ecology, and teaching. Accurate technique also supports comparison among specimens and long-term curation in biological collections.

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Education

JoVE Core - Nursing

Handwashing II: Pre-procedure and Initial Procedure Steps

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2023

The pre-procedure steps of handwashing include removing jewelry and rolling up sleeves. However, many organizations allow staff to wear wedding rings. The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail paint...

Handwashing III: During the Procedure and Post-Procedure Steps

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2023

To wash hands properly, follow these steps: Wet the hands. Use enough soap to cover all surfaces of the hands. Rub both hands palm to palm. Rub the back of the hand: Use the right palm over the left dorsum or back of the left hand with interlaced fingers, then switch hands. Rub palm to palm with fingers interlaced. With fingers interlocked, rub the backs of the fingers of the opposite hands. Rub the left thumb clasped in the right palm in a circular motion and repeat with the other...

Tracheostomy Care I: Pre-procedural Steps

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2025

A tracheostomy is a surgical technique that involves making an incision in the neck to provide access to the trachea. It is frequently used in medical conditions such as airway obstruction and prolonged mechanical ventilation. Effective nursing management is crucial for the long-term success of a tracheostomy. Required Equipment The equipment necessary for tracheostomy care includes: Disposable and sterile gloves Goggles and mask or face shield Additional PPE, as indicated Sterile normal...

Tracheostomy Suctioning I: Pre-Procedural Steps

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2025

Tracheostomy suctioning is a critical procedure healthcare professionals perform to maintain a patent airway in patients with a tracheostomy tube. This procedure is necessary when secretions accumulate in the airway, causing respiratory distress. Here is a step-wise procedural guide for performing tracheostomy suctioning using an open system. Equipment Required First, gather all necessary equipment: a sterile suction catheter, a sterile disposable container, sterile gloves, a towel or...

Research

JoVE Journal - Biology

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

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Cited by 93 •

2013

A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.

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