Plant Leaf Extraction
Plant Leaf Extraction - Related Videos
05:43
Extraction of Genomic DNA from Plant Leaf Tissue Infected with Pathogenic Bacteria
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological analysis.
10:37
Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts
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Cited by 6 •
2016
The design-of-experiments procedure presented here allows the evaluation of different flocculants in terms of their ability to aggregate dispersed particles in plant extracts, thus reducing turbidity and the costs of downstream processing.
11:50
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Cited by 47 •
2015
Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.
02:26
Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding
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2025
This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.
08:14
LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
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Cited by 93 •
2013
Demonstration of key methods for high throughput leaf measurements. These methods can be used to accelerate leaf phenotyping when studying many plant mutants or otherwise screening plants by leaf phenotype.
FAQs
The solvent or buffer helps determine which cellular compounds remain in the liquid extract after leaf tissue is disrupted. Different extraction conditions may favor recovery of pigments, nucleic acids, proteins, or other metabolites. Researchers therefore select conditions according to the compound they want to identify or measure, making the extract more suitable for the intended biological analysis.
Homogenization physically disrupts leaf tissue so that cellular compounds can move into the selected solvent or buffer. More complete disruption can improve contact between the tissue and liquid phase, whereas insufficient disruption may leave compounds in the solid material. This step directly affects the composition and usefulness of the final extract for biochemical or physiological studies.
Recovery depends mainly on the extraction conditions used after the leaf tissue is collected and disrupted. The selected solvent or buffer, together with the way the material is processed and separated, influences which compounds enter the liquid fraction. Matching those conditions to the target compound allows researchers to obtain extracts appropriate for identification or measurement.
Filtration or centrifugation removes disrupted tissue and other solid debris from the liquid fraction. This produces a cleaner extract for subsequent identification or measurement and helps distinguish compounds released from the leaf from the remaining physical material. The separation step is therefore important when the analysis requires a liquid sample with reduced solid interference.
A typical workflow begins by collecting the leaves and homogenizing the tissue. The disrupted material is then mixed with a suitable solvent or buffer to release cellular compounds. Finally, the mixture is separated from solid debris through filtration or centrifugation. The resulting liquid extract can then be used for biological analysis of the recovered compounds.
Researchers use leaf extracts when they need to examine compounds associated with plant function rather than study intact tissue alone. Applications described for this method include investigations of plant physiology, photosynthesis, genetics, disease responses, and biochemical composition. The approach provides material for identifying or measuring cellular compounds involved in these biological processes.
Researchers can prepare extracts from leaves exposed to different environmental conditions or collected at different developmental stages, then compare their recovered compounds. Differences in pigments, nucleic acids, proteins, or other metabolites can provide biochemical evidence of changes in plant function. This makes extraction useful for connecting external conditions or development with cellular composition.