Plasma Fluorescence
Plasma Fluorescence - Related Videos
08:42
How to Ignite an Atmospheric Pressure Microwave Plasma Torch without Any Additional Igniters
0 Views •
Cited by 26 •
2015
This movie shows how an atmospheric plasma torch can be ignited by microwaves with no additional igniters and provides a stable and continuous plasma operation suitable for plenty of applications.
08:36
An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation
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Cited by 20 •
2016
An experimental setup was created for the helium-operated kHz frequency plasma jet. The setup includes a cage for the plasma power supply and jet and an in-house built reactor to monitor plasma-induced reactive species without the interference of the ambient atmosphere.
07:26
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Cited by 9 •
2016
PI(4,5)P2 regulates various cellular functions, but its nanoscale organization in the cell plasma membrane is poorly understood. By labeling PI(4,5)P2 with a dual-color fluorescent probe fused to the Pleckstrin Homology domain, we describe a novel approach to study the PI(4,5)P2 spatial distribution in the plasma membrane at the nanometer scale.
04:34
An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency
0 Views •
2025
This video demonstrates an assay to study the plasma membrane resealing efficiency in mammalian cells. The cells are exposed to a bacterial pore-forming toxin, which forms pores in the cell membrane. In the presence of extracellular calcium ions, the pore causes calcium influx, inducing membrane-resealing events. The resealing efficiency is assessed using DNA-binding propidium iodide dye, which only enters cells without a resealed membrane.
06:46
Applying X-ray Imaging Crystal Spectroscopy for Use as a High Temperature Plasma Diagnostic
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Cited by 4 •
2016
X-ray spectra provide a wealth of information on high temperature plasmas. This manuscript presents the operation of a high wavelength resolution spatially imaging X-ray spectrometer used to view hydrogen- and helium-like ions of medium atomic number elements in a tokamak plasma.
FAQs
The excitation wavelength determines which absorbing molecules are promoted to excited electronic states, while the emitted light appears at a longer wavelength after partial energy loss. Selecting and examining these wavelengths helps distinguish fluorescence responses associated with different molecular constituents. The resulting spectral profile can therefore provide information about the composition and molecular environment of a biological plasma sample.
Endogenous fluorophores are naturally fluorescent molecules already present in the plasma, whereas added fluorescent probes introduce a signal from a selected external compound. Their signals can provide complementary information: native fluorescence reflects existing biological constituents, while probe-based fluorescence can support examination of particular molecular features. This distinction helps researchers interpret whether an observed response originates from the sample or an added reagent.
Fluorescence intensity reflects the strength of the emitted signal, while the spectral profile describes how that signal is distributed across emission wavelengths. Together, these characteristics can change with molecular composition and environment. Examining both rather than intensity alone gives a more informative view of plasma constituents and supports biochemical interpretation of differences between biological samples.
Analysis can help characterize proteins, metabolites, and other biomolecules in blood-derived plasma. Because fluorescence intensity and spectral features reflect molecular composition and environment, measurements may reveal differences among samples or changes associated with biological conditions. This makes the approach useful for biochemical assays and for investigating molecular patterns that may contribute to disease-related studies.
Biology researchers can apply the technique to biochemical assays, disease-related investigations, physiological monitoring, and biomarker research. In these settings, fluorescence measurements provide molecular information from blood-derived samples rather than relying only on visible sample characteristics. The approach can support comparisons among biological states and help identify fluorescence patterns relevant to changing physiology or disease-associated processes.
A basic analysis begins by exciting the biological plasma or a sample containing an added fluorescent probe, then examining the resulting emitted light. Researchers consider the emission intensity and spectral profile in relation to the sample’s molecular composition and environment. Interpreting these measurements can support characterization of biomolecules, biochemical testing, and searches for patterns associated with physiological change.