Platelet Fragment Depletion
Platelet Fragment Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
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Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
08:32
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
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Cited by 133 •
2009
Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.
02:31
Habitat Fragmentation
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2020
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition. Perforation and dissection often occur during the initial stages of...
09:13
Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Cited by 5 •
2017
We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.
03:58
Purification and Activation of Platelets from Murine Whole Blood
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2025
In this video, we describe a protocol for purifying platelets from murine whole blood and their subsequent activation with the platelet agonist thrombin. When analyzed with flow cytometry, the purified resting platelets are characterized based on the expression of their cell-surface marker CD41, whereas purified activated platelets co-express both the surface markers CD41 and CD62P.
FAQs
The separation relies on measurable differences between platelet-derived material and the desired target. Controlled centrifugation uses differences in density, filtration uses differences related to size, and selective immunomagnetic capture uses surface markers. Selecting the appropriate physical or molecular distinction helps remove platelet fragments while preserving the target population needed for downstream biological analysis.
Residual platelet material can introduce platelet-associated signals into samples that are intended to represent other cells or analytes. This contamination may complicate interpretation of gene-expression profiles, signaling analyses, and cell-cell interaction studies. Removing it improves sample purity, helping measured changes more accurately reflect the target biological material rather than platelet-derived contributions.
These approaches separate unwanted material by different principles. Controlled centrifugation exploits density, filtration exploits size, and selective immunomagnetic capture targets surface markers on platelet-derived material. The most suitable method depends on which distinguishing property is available in the sample and whether the priority is physical separation or selective removal based on molecular identity.
Effectiveness depends on how clearly platelet fragments differ from the target in size, density, or surface-marker profile. The selected separation approach must remove residual platelet material without compromising the target cells or analytes. A suitable method therefore supports both improved purity and reliable recovery of material required for later measurements or functional assays.
A basic workflow starts by identifying the target cells or analytes and the distinguishing property of the platelet material. The sample is then processed with controlled centrifugation, filtration, or selective immunomagnetic capture. The resulting preparation is used for downstream analysis, with the chosen separation strategy matched to the sample’s required purity and target preservation.
This preparation is particularly useful when platelet contamination could alter interpretation of downstream results. Researchers may apply it before flow-cytometry measurements, molecular analyses, cell-cell interaction studies, or functional assays. It is also relevant when gene-expression or signaling data must be attributed confidently to the target biological material rather than residual platelet components.
Depleting platelet-derived fragments produces cleaner cell suspensions and reduces platelet-associated interference. In flow cytometry, this supports clearer analysis of the intended cellular population; in molecular measurements, it helps limit contributions from residual platelet material. These improvements can make biological differences easier to interpret and strengthen conclusions from downstream experiments.