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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

DOI:
10.3791/55525-v

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09:13 min

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April 06, 2017

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, , ,
1Department of Pathology and Immunology,University of Geneva, 2Division of Angiology and Haemostasis & Geneva Platelet Group,Geneva University Hospitals

Chapters

  • 00:05Title
  • 00:57Buffer Preparation
  • 02:30Blood Collection
  • 03:17Preparation of Human Washed Platelets
  • 05:47Aggregometry
  • 07:39Results: Anti-platelet Activity of Brilliant Blue FCF
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.

Tags

Keywords: TurbidimetryHuman Washed PlateletsPannexin1-inhibitorBrilliant Blue FCFCollagen-induced AggregationPlatelet BiologyPlatelet FunctionACD SolutionTyrode’s BufferTA BufferPlatelet-rich PlasmaCentrifugationLight Transmission Aggregometry

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