Pollen Analysis
Pollen Analysis - Related Videos
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
07:07
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
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2023
An improved method to measure pollen hydration profiles in Arabidopsis thaliana is described here. The new method offers higher resolution, is noninvasive, and is highly reproducible. The protocol represents a new tool for a finer dissection of the processes that regulate the early stages of pollination.
07:19
A 3D Printed Pollen Trap for Bumble Bee (Bombus) Hive Entrances
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Cited by 7 •
2020
We present a non-lethal and automated mechanism to collect pollen from bumble bee (Bombus) workers returning to a hive. Instructions for producing, preparing, installing and using the devices are included. By using 3D-printed objects, modification to the design was timely, efficient and allowed for quick turnaround for testing.
06:45
Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
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Cited by 4 •
2023
Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.
FAQs
Identification depends on the durable outer wall of a grain, whose shape, size, and surface patterns can be compared with reference material. This comparison allows analysts to associate grains with plant groups rather than relying on appearance alone. Microscopic examination makes those fine structural features available for consistent recognition across environmental or biological samples.
Sediment cores add a time dimension to pollen analysis. As analysts examine successive layers, each layer contributes a position in an ordered sequence, allowing changes in the associated vegetation record to be followed through time. This chronological structure is why the approach supports paleoenvironmental reconstruction and climate research, rather than only describing plants present in one contemporary sample.
When identified grains are considered as an assemblage, their combined pattern can be related to a habitat or location. This broader view is useful because pollen analysis does not depend only on one grain; it uses the set of observed features to support ecological interpretation and comparisons among samples. Assemblages therefore connect microscopic observations with larger biological settings.
A basic workflow for pollen analysis begins with an environmental or biological sample, followed by microscopic examination of the grains. Analysts then compare observed shape, size, and surface patterns with reference material to identify plant groups. When samples come from successive sediment-core layers, the identifications can be organized into a chronological vegetation record for later interpretation.
Pollen analysis supports several biological research goals: ecological surveys can document plant representation, while paleoenvironmental reconstruction uses records from successive sediment layers to examine past vegetation. Climate research can interpret vegetation changes through those records, and distribution studies can connect identified pollen with where plant groups occur. The method therefore links microscopic observations to broader environmental patterns.
In agriculture and forensic investigation, pollen assemblages can provide location-related evidence. Agricultural studies may use them to characterize the plant context surrounding a setting, while forensic work can compare assemblages with habitats or locations. These applications rely on the ecological signal represented by the observed pollen types, so interpretation requires relating the assemblage to appropriate reference material.