Polyethyleneimine
Polyethyleneimine - Related Videos
09:36
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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Cited by 21 •
2019
We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.
05:50
Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and Functional Testing
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2026
This protocol presents Rayleigh breakup as a mild process for aerosolizing non-viral mRNA vectors. Regional deposition for aerosolized mRNA vectors is evaluated by the Alberta Idealized Nasal Inlet (AINI) model. Green fluorescence protein (GFP) expression in human lung cells represents maintenance of physicochemical and biological functions of post-aerosolized mRNA vectors.
02:53
A Technique to Generate Monoclonal Antibodies from Antigen-Specific B Cells
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2025
The video demonstrates a technique for generating monoclonal antibodies from antigen-specific B cells. Eukaryotic expression vectors encoding the heavy and light chains of antibodies, cloned from isolated antigen-specific B cells, are introduced into mammalian cells using polyplexes. Once inside the cells, these vectors undergo translation, and the resulting heavy and light chain peptides assemble into antibodies, which are subsequently secreted by the cells.
07:28
Synthetic Biology
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2023
This video presents synthetic biology and its role in bioengineering. Synthetic biology refers to the methods used to genetically modify organisms in order to make them capable of producing large quantities of a product. This product could be a protein that the cell already makes or a new protein that has been encoded in a newly-inserted DNA sequence. Here, we discuss how an organism's genetic material is modified using transformation or transfection. Then, the process is shown in the...
FAQs
PEI’s multiple protonatable amines create electrostatic attraction with negatively charged DNA or RNA. Primary, secondary, and tertiary amines provide the chemical sites for this association, allowing the polymer to condense nucleic-acid cargo and shield it. That charge-based organization supports later delivery steps in a biological delivery context.
After internalization, PEI’s protonatable groups can buffer the surrounding environment within endosomal compartments. That buffering is relevant because it can support release of associated nucleic-acid cargo from those compartments, a step connecting intracellular processing with access to the cell. The mechanism therefore complements the initial electrostatic condensation and uptake functions.
Delivery performance depends on more than the presence of PEI. Polymer size, branching, charge, and dose can each be tuned, and these variables influence the balance between delivery efficiency and cytotoxicity. Consequently, a formulation that improves nucleic-acid transfer may still require adjustment if its dose or polymer properties produce unacceptable effects on cells.
An optimization workflow begins by varying PEI formulation properties and dose rather than treating the polymer as a fixed reagent. Researchers can compare resulting delivery efficiency with observed cytotoxicity, then select conditions that provide a useful balance. This approach is important because formulation variables can affect both delivery performance and cellular tolerance.
PEI-based formulations support several gene-transfer workflows: transient gene expression, RNA delivery, and production of recombinant proteins. These uses span introducing nucleic-acid instructions into cells and enabling expression-based experiments. The method is therefore relevant when researchers need a delivery tool for expression studies or protein-production work.
Delivery efficiency alone does not establish that a PEI formulation is suitable. Researchers also need to consider cytotoxicity, because the polymer’s dose, charge, size, and branching can shift the balance between successful cargo delivery and harmful cellular effects. Evaluating both outcomes helps distinguish a productive formulation from one that performs well only at an unacceptable biological cost.