Post-translational Regulation
Post-translational Regulation - Related Videos
01:29
Translational Regulation
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2025
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
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Cited by 8 •
2014
We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.
05:56
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
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Cited by 1 •
2022
The present protocol uses a biomolecular simulation package and describes the molecular dynamics (MD) approach for modeling the wild-type caspase and its mutant forms. The MD method allows for assessing the dynamic evolution of the caspase structure and the potential effect of mutations or post-translational modifications.
04:25
Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene
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2025
This video demonstrates an in vivo assay in bacteria to study the interaction of RNA-binding proteins (RBPs) with RNA. The bacterial cells are transformed with two plasmid constructs — a binding-site plasmid encoding an mRNA containing a fluorescent reporter gene downstream of an RBP-binding site — while an RBP plasmid expresses the RBP proteins under the control of an inducer. Upon inducer-mediated increase in RBP production, the RBPs bind to the mRNA, resulting in translational repression of...
FAQs
Reversible changes can be added and removed, allowing protein behavior to shift as cellular conditions change. Irreversible changes, such as proteolytic cleavage, permanently process the protein or remove a regulatory segment. This distinction affects whether regulation functions as a temporary switch or a lasting alteration in protein activity, location, stability, or interactions.
Regulatory enzymes can add chemical groups, remove them, or process regulatory sequences within a protein. These actions may change the protein’s conformation, which influences its activity and binding behavior. They can also affect stability or cellular trafficking, allowing the same protein to perform different roles depending on which regulatory change occurs.
Its effects extend to where a protein is located, how long it remains stable, and which molecules it can interact with. By coordinating these properties, cells can adjust signaling, metabolism, cell-cycle progression, and responses to environmental conditions. Regulation therefore changes both the behavior of individual proteins and the broader cellular processes they support.
These changes represent distinct ways to modify protein behavior after synthesis. Phosphorylation, acetylation, and ubiquitination involve adding chemical groups, whereas proteolytic cleavage processes the protein itself. Depending on the modification, the outcome may involve altered conformation, binding, stability, activity, or trafficking, so the same general regulatory principle can produce different cellular effects.
Mapping these regulatory changes can reveal how abnormal protein control contributes to cancer, neurodegeneration, or immune disorders. Such analysis connects altered protein activity, stability, interactions, or localization with disease-related cellular behavior. The resulting regulatory patterns may support biomarker discovery by identifying informative molecular changes associated with pathological states.
Because these mechanisms can control protein activity, stability, interactions, and cellular trafficking, they provide biologically meaningful points for understanding or influencing cellular behavior. Mapping the changes associated with disease can identify regulatory patterns relevant to treatment development. This context helps connect molecular regulation with therapeutic strategies without requiring changes to gene transcription.