Mature Mirna
Mature Mirna - Related Videos
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
0 Views •
2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
0 Views •
2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
0 Views •
2023
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
0 Views •
2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
01:28
Maturation of Endosomes
0 Views •
2023
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB). Changes in location The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
FAQs
Each factor performs a distinct processing or delivery step. Drosha and DGCR8 cut the primary transcript into a precursor hairpin, Exportin-5 moves that precursor into the cytoplasm, and Dicer produces the short duplex. Argonaute then accepts the guide strand in RISC, positioning the regulatory sequence for target recognition.
Guide-strand loading determines which nucleotide sequence the regulatory complex presents to potential targets. Once incorporated into Argonaute-containing RISC, that strand directs recognition of partially complementary regions in mRNAs. The loaded sequence therefore links miRNA processing to regulatory specificity, influencing which transcripts can undergo reduced translation or destabilization.
Partial rather than complete complementarity is central to animal miRNA regulation. It allows the guide-containing complex to recognize target sites without requiring an exact match across the entire sequence. Sites often occur in a messenger RNA’s 3′ untranslated region, where recognition can reduce translation or promote mRNA destabilization after transcription.
Start with the primary transcript, then follow Drosha-DGCR8 cleavage, Exportin-5 transport, Dicer processing, and guide-strand loading into Argonaute-containing RISC. The investigation can then connect the resulting complex with partially complementary mRNA sites and assess the two stated regulatory outcomes: reduced translation or mRNA destabilization.
Because these molecules connect gene transcripts with post-transcriptional regulation, researchers can examine how particular miRNA-guided interactions influence cellular programs. In biology, this framework is relevant to functional genetics because it links a noncoding RNA product to changes in target-mRNA handling, helping investigate mechanisms underlying development, physiology, and disease.
Their defined processing pathway and ability to regulate target mRNAs make mature miRNAs useful subjects for translational investigation. Biomarker studies can focus on their relationship to disease biology, while therapeutic research can examine their potential role in influencing gene-expression control. These areas represent research directions rather than guaranteed clinical applications.