Proteins Amino Acids

Proteins are biological macromolecules built from amino acids, and they perform essential structural, catalytic, transport, signaling, and regulatory functions in cells. Each amino acid contains an amino group, a carboxyl group, and a variable side chain; cells join amino acids through peptide bonds, and the resulting sequence folds into a three-dimensional structure that determines protein activity. In biology, studying proteins and amino acids clarifies how genes produce functional molecules and how molecular changes affect cells and organisms. These concepts support research in enzymology, genetics, nutrition, biotechnology, disease mechanisms, and therapeutic development.

Proteins Amino Acids - Related Videos

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JoVE Core - Biology

Proteins and Amino Acids

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2026

Proteins are chains of amino acids that are connected by peptide bonds and folded into a 3-dimensional structure. The side chains of individual amino acid residues determine the interactions among amino acid residues, and ultimately the folding of the protein. Depending on the length and structural complexity, chains of amino acid residues are classified as oligopeptides, polypeptides, or proteins.Amino Acid Residues Are the Building Blocks of ProteinsAn amino acid is a molecule that contains a...

Research

JoVE EoE - Assay Techniques

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

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2025

This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.

Amino acids

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2026

Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible for...

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

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Cited by 6 •

2016

Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.

Research

JoVE Journal - Biology
Free Sample

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

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Cited by 16 •

2016

An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.

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