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Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
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Neuroscience
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JoVE Journal Neuroscience
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

DOI:
10.3791/53818-v

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08:20 min

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March 28, 2016

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, ,
1Department of Neuroscience, School of Medicine,Tufts University, 2Molecular Neurobiology Laboratory,The Salk Institute for Biological Studies, 3Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute,University of California, San Francisco

Chapters

  • 00:05Title
  • 00:42Culture and Calcium Phosphate Transfection of Rat Hippocampal Primary Neurons
  • 03:49Whole-cell Recording with Light Activation
  • 06:19Results: PIRK Expression and Activation in Rat Hippocampal Neurons and Mouse Neocortex
  • 07:26Conclusion

Summary

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Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.

Tags

Optical ControlNeuronal ProteinGenetically EncodedUnnatural Amino AcidNeuronsHippocampal NeuronsCalcium Phosphate TransfectionNeuronal SignalingBrain FunctionSpatial Temporal Resolution

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