Rapid Field Detection
Rapid Field Detection - Related Videos
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
08:50
A Nonsequencing Approach for the Rapid Detection of RNA Editing
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2022
Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
11:02
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
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Cited by 43 •
2015
Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.
06:58
Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
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Cited by 2 •
2010
Magnetic modulation biosensing system is utilized to rapidly, sensitively and simply detect biological assays, such as DNA molecules and proteins.
03:44
A Rapid Method to Confine and Safely Handle Bees in the Field
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Cited by 1 •
2024
We demonstrate a tested method for safely handling field-collected bees. This method allows swift manipulation, identification, genetic sampling, and confirmation of plant-insect interactions via pollen collected while sampling. Easily adaptable, this approach offers a cost-effective, non-lethal means to study rare insect groups.
FAQs
The process links recognition of a biological target to a measurable output. Depending on the platform, that output may be a visible color change, fluorescence, or an electrical response. The signal gives investigators an immediate indication that the target or environmental indicator has been recognized, allowing observations to be made close to the sampling site rather than after centralized laboratory processing.
These signal types provide different ways to observe the outcome of target recognition. A color change can support direct visual interpretation, while fluorescence or an electrical response can provide instrument-based measurements. Their common role is to translate biological interactions into detectable outputs, making portable assays adaptable to pathogen surveillance, ecological monitoring, and safety testing.
Nucleic acid amplification is useful when increasing the amount of target material can improve detection sensitivity. In field platforms, amplification must occur under controlled conditions so that the resulting signal remains interpretable. This capability extends the range of biological targets that can be examined outside centralized laboratories, supporting earlier investigation of possible pathogens or other biological signals.
A typical workflow begins with collecting a biological or environmental sample at or near the study site. The sample is then exposed to a portable assay or instrument that recognizes the target, with nucleic acid amplification included when appropriate. Finally, the system produces a visible, fluorescent, or electrical signal that can inform a timely field decision.
Field testing relies on portable assays and instruments designed to operate near the sampling location. Their outputs may be visual, such as a color change, or instrument-derived, such as fluorescence or an electrical response. The combination of portable equipment and measurable signals allows biological observations to be generated where samples are collected, reducing dependence on centralized laboratory access.
Researchers may choose this approach when decisions depend on obtaining information quickly or when sampling occurs far from a centralized laboratory. Its applications include pathogen surveillance, ecological monitoring, food and water safety, and biodiversity studies. By shortening the distance between sampling and measurement, the approach can support outbreak response and field-based biological research.
Portable assays and instruments can extend biological testing to settings where access to centralized laboratories is limited. In these environments, near-site measurements can provide timely information about pathogens, environmental conditions, or biological diversity. The resulting access supports surveillance, safety assessment, and ecological studies while helping investigators make decisions closer to where biological changes are occurring.