Rna-induced Silencing Complex
Rna-induced Silencing Complex - Related Videos
10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
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Cited by 159 •
2011
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
02:12
Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing
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2026
Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...
02:57
Targeted siRNA Delivery to Neurons for Prion Protein Silencing Using Liposomal Complexes
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2025
Source: Bender, H. R., et.al. Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes. J. Vis. Exp. (2016)This video demonstrates the targeted delivery of prion protein (PrP) siRNA to neurons using liposomal siRNA-peptide complexes (LSPCs). Cationic liposomes electrostatically bind PrP-targeting siRNA, which is further stabilized by the RVG-9r peptide for receptor-mediated transport across the blood-brain barrier. Once inside the brain, LSPCs undergo endocytosis in...
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
08:36
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
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Cited by 94 •
2015
Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.
FAQs
Sequence complementarity gives RISC its targeting specificity. The guide strand carries sequence information that enables base pairing with a matching messenger RNA, directing the complex toward that transcript rather than unrelated messages. This recognition step connects the presence of a particular small RNA with selective regulation of gene expression.
Argonaute provides the central protein component that holds the guide strand inside the complex. Once the guide pairs with a target transcript, Argonaute-associated activity can promote cleavage, destabilization, or translational repression. Its position within RISC links RNA recognition to molecular events that reduce expression of the selected gene.
RISC does not silence every target through a single molecular outcome. Depending on the interaction with the target transcript, regulation may involve direct mRNA cleavage, increased transcript destabilization, or reduced translation. Distinguishing these outcomes matters because a measured decrease in gene expression can arise from transcript loss, impaired message stability, or blocked protein production.
RISC-mediated silencing contributes to development, cellular identity, and responses to genomic or viral threats. In these contexts, selective control of messenger RNAs can help cells alter gene-expression programs or respond to potentially harmful genetic material. This broader role places the complex at the intersection of routine biological regulation and defense-related processes.
Researchers use targeted knockdown experiments to reduce expression of a selected gene and examine the resulting biological consequences. A small RNA provides the sequence information that directs RISC toward the corresponding messenger RNA. This approach helps connect reduced expression of a chosen transcript with the gene function under investigation.
RISC provides a mechanism for considering whether selected messenger RNAs can be regulated through sequence-directed small RNAs. In therapeutic research, this supports investigation of strategies designed to reduce expression of specific transcripts. The relevant outcome is not simply RNA delivery, but whether targeted cleavage, destabilization, or translational repression produces useful gene regulation.