Seedling Microbiota
Seedling Microbiota - Related Videos
02:29
Measuring Immune-Induced Inhibition of Seedling Growth
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2025
This video demonstrates the seedling growth inhibition assay in the Arabidopsis thaliana plant. The impact of increased concentration of immune peptide elicitor on seedling growth is determined.
10:00
Isolation of Tomato Seed-Associated Microbiota Through Controlled Germination and Comparative Culturing
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2026
This protocol combines standardized seed surface sterilization with controlled germination to enable the reproducible isolation of tomato seed-associated microbiota during the seed-to-seedling transition. The approach enhances recovery of culturable microorganisms that are underrepresented in dry seeds and supports comparative culturomic studies during early seedling development.
02:30
Seedling Flood Assay for Screening Bacterial Resistance in Tomatoes
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2025
Source: Hassan, J. A., et al. High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay. J. Vis. Exp. (2020).This video demonstrates a method to screen for bacterial resistance in different tomato strains using ten-day-old seedlings inoculated with a bacterial suspension. It explains how bacteria invade through stomata, deliver effector proteins to suppress plant immunity, and how plant responses differ between susceptible and resistant...
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
06:56
Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
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Cited by 6 •
2021
To obtain an axenic insect, its egg surface is sterilized, and the hatched larva is subsequently reared using axenic leaves. This method provides an efficient way for axenic insect preparation without administering antibiotics or developing an artificial diet, which can also be applied to other leaf-eating insects.
FAQs
Community assembly depends on multiple transmission routes. Microbes may arrive through the seed itself, soil, water, or nearby surroundings. These inputs shape which organisms encounter the developing plant, while competition for nutrients and interactions among microbes further influence community composition. Studying these routes helps explain why early microbial partnerships differ across seedlings and how establishment conditions can affect later growth and survival.
Effects on early development can arise through several linked mechanisms. Some microbes release compounds that promote growth, while others alter plant hormone signaling, the chemical communication system that regulates development. Microbial competition for nutrients can also change which organisms persist around the seedling. Together, these processes connect community composition with plant growth rather than treating microbes as passive occupants.
Seedling microbiota can influence pathogen resistance by activating or suppressing plant defenses. The same community-level perspective also considers competition for nutrients and interactions among microorganisms, because these processes affect which microbial partners remain associated with the plant. Examining these links helps biology researchers distinguish microbial effects on defense from broader effects on early plant establishment and survival.
Microbial partnerships formed during seedling establishment can contribute to the plant’s ability to tolerate environmental stress. Their effects may involve growth-promoting compounds, altered hormone signaling, or changes in defense activity. Studying these relationships connects early microbial colonization with plant survival and helps researchers investigate why some seedlings maintain development under challenging conditions.
A study should consider microorganisms transmitted by the seed, soil, water, and surrounding environments rather than focusing on a single source. Researchers can then relate these origins to microbial competition, plant signaling, defense responses, and early growth. This approach helps identify how environmental exposure and inherited microbial associations jointly shape the developing plant community.
Investigations can reveal how plants establish beneficial microbial partnerships and how those partnerships relate to early growth and survival. They can also clarify links between microbial communities, hormone signaling, plant defenses, environmental stress tolerance, and pathogen resistance. These outcomes provide biological context for understanding plant development as an interaction between the seedling and its microbial associates.
Findings can guide the design of microbial inoculants, which are introduced microbial partners intended to support plant performance. This research also contributes to sustainable agriculture by linking microbial relationships with crop resilience, growth, stress tolerance, and pathogen resistance. The broader goal is to apply knowledge of early plant-microbe interactions to improve crop establishment and performance.