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JoVE Journal
Biology

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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
 

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana

Article DOI: 10.3791/1149
August 17th, 2009

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Summary August 17th, 2009

This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.

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