Shielding Targeting
Shielding Targeting - Related Videos
08:14
Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
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Cited by 9 •
2018
The protocol described here enables researchers to specifically modify adenovirus capsids at selected sites by simple chemistry. Shielded adenovirus vectors particles and retargeted gene transfer vectors can be generated, and vector host interactions can be studied.
01:14
Diamagnetic Shielding of Nuclei: Local Diamagnetic Current
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2024
An applied magnetic field causes the electrons present in the molecule to circulate, setting up a local diamagnetic current within the molecule. The local diamagnetic current arising from circulating sigma-bonding electrons induces a magnetic field, Blocal that opposes the applied magnetic field, B0. The effective magnetic field experienced by these nuclei is given by the difference between the applied and local magnetic fields in a phenomenon called local diamagnetic shielding. Essentially,...
18:57
Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
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Cited by 12 •
2009
Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.
08:40
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Cited by 1 •
2012
The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.
05:56
Construction of a Compact Low-Cost Radiation Shield for Air-Temperature Sensors in Ecological Field Studies
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Cited by 8 •
2018
With the advent of small, low-cost environmental sensors, it is now possible to deploy high-density networks of sensors to measure hyper localized temperature variation. Here, we provide a detailed methodology for constructing a compact version of a previously described custom-fabricated radiation shield for use with inexpensive thermochrons.
FAQs
The design must balance two potentially competing outcomes: strong shielding can limit unwanted interactions and premature clearance, while accessible ligands must remain available for receptor binding. If the protective layer obscures recognition elements, selective attachment may decrease; if protection is insufficient, nonspecific protein adsorption and immune recognition can increase. This balance influences circulation stability and subsequent cellular uptake.
Hydrophilic polymers or biomolecules form the protective surface layer in Shielding Targeting. This layer helps reduce nonspecific protein adsorption, immune recognition, and premature clearance from circulation. By limiting these interactions, the delivery system can remain more stable before reaching its intended cells. The shielding component therefore supports persistence while the targeting component provides selective recognition.
Targeting ligands promote uptake by binding receptors associated with the intended cell type. After this selective interaction occurs, the cell can internalize the carrier through receptor-mediated uptake. This mechanism connects molecular recognition with cargo entry, helping direct drugs, nucleic acids, or imaging agents toward selected cells rather than relying only on nonspecific distribution.
The two components must be coordinated because each addresses a different delivery problem. Shielding limits recognition by proteins and immune processes, whereas exposed ligands enable recognition by receptors on selected cells. A design that favors only protection may reduce cellular binding, while one that favors only ligand exposure may increase unwanted interactions. Their coordination supports more selective delivery.
Design begins by matching the protective layer and exposed recognition elements to the intended delivery goal. Researchers consider whether the system should resist protein adsorption, immune recognition, and premature clearance while still allowing ligand-receptor binding. They also select a suitable cargo category, such as a drug, nucleic acid, or imaging agent, based on the desired biological or diagnostic use.
Shielding Targeting is applied to delivery systems for therapeutic and diagnostic cargo. In nanomedicine and molecular biology, the approach supports transport of drugs, nucleic acids, and imaging agents toward specific cells. Its relevance comes from the possibility of improving delivery precision, reducing off-target effects, maintaining circulation stability, and increasing treatment efficiency through more selective cellular uptake.