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Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
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JoVE Journal Biology
Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

DOI:
10.3791/58480-v

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08:14 min

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October 26, 2018

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, ,
1Center of Biomedical Education and Research,University Witten/Herdecke

Chapters

  • 00:04Title
  • 01:31Coupling Moieties: Storage and Preparation
  • 02:43Amplification, Purification, and Chemical Modification of Ad Vectors
  • 06:36Results: Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol described here enables researchers to specifically modify adenovirus capsids at selected sites by simple chemistry. Shielded adenovirus vectors particles and retargeted gene transfer vectors can be generated, and vector host interactions can be studied.

Tags

Keywords: Gene TherapyOncolysisGenetic VaccinationAdenovirusGene Transfer VectorCapsid ModificationChemical ModificationVector-host InteractionVirus LabelingVirus Tracking

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