Shigella Infection
Shigella Infection - Related Videos
02:51
Stress Granule Formation During Shigella Infection of Cancerous Epithelial Cells
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2026
Source: Vonaesch, P. et. al., Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells. J. Vis. Exp. (2017)This video demonstrates the infection of epithelial cells with the pathogenic bacterium Shigella flexneri and the resulting host cellular response. Bacterial effector proteins disrupt host translation, triggering the formation of membrane-less organelles called stress granules, which reflect the cell's adaptive response to infection.
04:56
Epithelial Cell Infection Analyses with Shigella
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Cited by 1 •
2024
The present protocol describes infection assays to interrogate Shigella adherence, invasion, and intracellular replication using in vitro epithelial cell lines.
08:56
Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
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Cited by 4 •
2018
Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.
04:22
Intracellular Replication Assay for Shigella in Human Colonic Epithelial Cells
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2026
Source: Poore, K., et al. Epithelial Cell Infection Analyses with Shigella. J. Vis. Exp. (2024)This video demonstrates an in vitro assay to assess Shigella invasion and intracellular replication in human colonic epithelial monolayers. It highlights steps for infection, antibiotic selection, host cell lysis, and recovery of intracellular bacteria for downstream analysis.
12:52
Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
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Cited by 14 •
2014
To counteract pathogen dissemination, host cells reorganize their cytoskeleton to compartmentalize bacteria and induce autophagy. Using Shigella infection of tissue culture cells, host and pathogen determinants underlying this process are identified and characterized. Using zebrafish models of Shigella infection, the role of discovered molecules and mechanisms are investigated in vivo.
FAQs
The type III secretion system functions as a bacterial delivery apparatus during Shigella infection. It enables the bacterium to manipulate processes inside host cells rather than relying only on extracellular growth. This activity helps explain how epithelial invasion, movement between neighboring cells, and the resulting inflammatory response are biologically connected.
Cell-to-cell spread matters because it allows Shigella to move through the intestinal epithelial layer after initial invasion. This behavior links a local interaction with one host cell to broader tissue involvement and intense inflammation. In biology research, examining this sequence helps clarify how bacterial movement and host responses contribute to disease.
Fecal-oral exposure makes transmission dependent on preventing contact with material contaminated by intestinal waste. That connection gives public-health studies a direct route from bacterial biology to sanitation: investigators can examine how transmission occurs and use those findings to support measures that reduce exposure and disease spread.
Studying Shigella infection gives biology researchers a model for connecting bacterial pathogenesis with host immune responses. By examining how invasion, manipulation of host-cell processes, cell-to-cell spread, and inflammation relate, they can investigate disease mechanisms rather than viewing symptoms in isolation. This context supports broader understanding of host-microbe interactions in the intestine.
Investigating the infection’s bacterial and host-cell interactions can provide biological insight for improved diagnostic methods. Diagnostic development is important because the disease involves intestinal symptoms linked to invasion and inflammation, while transmission through fecal-oral exposure gives public-health teams a reason to identify cases and understand how disease spreads.
Rising antibiotic resistance makes Shigella infection important for antimicrobial research because existing treatment strategies may become less reliable. Researchers must therefore connect bacterial pathogenesis with approaches for controlling disease while recognizing that resistance changes the scientific and clinical context. This concern also strengthens the need for improved diagnostics and vaccine development.
Understanding invasion, manipulation of host-cell processes, cell-to-cell spread, and immune responses identifies biological features that vaccine research must consider. The goal is not simply to address symptoms, but to use knowledge of pathogenesis and host defense to guide strategies that may help prevent infection or reduce its consequences.