Short Hairpin Rna
Short Hairpin Rna - Related Videos
03:47
Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro: A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression
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2025
In this video, we demonstrate a method to perform transduction of shRNA lentiviral vectors to obtain stable knockdown cell lines in immortalized hematopoietic stem and progenitor cells (iHSPCs).
06:12
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
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2022
Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.
01:23
RNA Interference
0 Views •
2019
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated. This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
07:55
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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Cited by 3 •
2023
Here, we present an optimized in vitro method to uncover, quantify, and validate protein interactors of specific RNA sequences, using total protein extract from human cells, streptavidin beads coated with biotinylated RNA, and mass spectrometry analysis.
06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
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Cited by 13 •
2014
Optical tweezers have been used to study RNA folding by stretching individual molecules from their 5’ and 3’ ends. Here common procedures are described to synthesize RNA molecules for tweezing, calibration of the instrument, and methods to manipulate single molecules.
FAQs
Once delivered into a cell, shRNA is processed into small interfering RNA, or siRNA. The resulting siRNA associates with the RNA-induced silencing complex, known as RISC, which uses sequence complementarity to locate the matching messenger RNA. This targeting can produce messenger RNA cleavage or translational repression, reducing expression of the selected gene.
Sequence complementarity allows the RNA-induced silencing complex to recognize the messenger RNA corresponding to the selected gene. This molecular matching directs the silencing machinery toward a specific transcript rather than acting broadly across cellular RNA. Consequently, the experiment can connect reduced expression of one gene with changes relevant to its biological function.
shRNA vectors enable cells to produce the hairpin molecule in a way that supports sustained gene knockdown. Continued production helps maintain the downstream silencing pathway after delivery, making the approach useful when researchers need to examine gene effects over an extended study period in cultured cells or animal models.
A basic study begins by selecting a gene whose expression will be reduced, followed by delivering an shRNA construct into cells or an animal model. Inside the target cells, processing generates siRNA that enters the RISC pathway. Researchers then interpret the resulting reduction in gene expression in relation to the biological question under investigation.
Researchers use shRNA in cultured cells and animal models when they need sustained reduction of a selected gene to examine its role. In functional genomics, this approach helps connect gene knockdown with biological effects. Using more than one experimental setting can also support investigation of gene behavior in different biological contexts.
By reducing expression of a selected gene, shRNA experiments can help investigate whether that gene contributes to disease mechanisms. The resulting functional information may support evaluation of the gene as a potential therapeutic target. Thus, shRNA links molecular gene silencing with questions about disease biology and target prioritization.