Spiroplasma Genus
Spiroplasma Genus - Related Videos
10:07
Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1
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Cited by 17 •
2018
We present a procedure for growing several strains of Magnetospirillum in two different types of growth media. Magnetospirillum gryphiswaldense strain MSR-1 is grown in both liquid and O2 concentration gradient semi-solid media while M. magneticum strain AMB-1 and M. magnetotacticum strain MS-1 are grown in liquid medium.
06:15
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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Cited by 3 •
2022
Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
08:56
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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2018
Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
08:27
Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies
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Cited by 33 •
2015
Rapid bioassessment protocols using benthic macroinvertebrates are often used to monitor and assess water quality. An efficient protocol involves collections of Chironomidae surface-floating pupal exuviae (SFPE). Here, techniques for field collection, laboratory processing, slide mounting, and identification of Chironomidae SFPE are described.
03:21
Isolation of Bacteriophages from a Soil Sample
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2026
Source: Cross, T., et al. An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates. J. Vis. Exp.(2015)This video demonstrates the isolation of bacteriophages from a soil sample. A soil sample is collected and suspended in a phage buffer and allowed to settle the sediment. The extract is filtered, and nutrients are added. A vacuum filtration is done to remove microbial contaminants. The filtrate with phages is aliquoted into flasks, and...
FAQs
The sterol-containing membrane provides the structural support that a rigid cell wall would otherwise supply. This arrangement allows cells to remain flexible while preserving their characteristic helical form. That flexibility is biologically important because it supports the bending and twisting movements associated with the genus and helps explain why Spiroplasma differs from bacteria built around rigid walls.
Spiroplasma moves by flexing or twisting its helical body rather than relying on a rigid outer framework. Changes in the shape of the flexible cell generate movement through the surrounding environment. Studying this mechanism helps connect cell architecture with bacterial behavior and provides a useful comparison with bacteria whose movement depends on different structural arrangements.
Many Spiroplasma species depend on host organisms for nutrients and transmission, making host relationships central to their biology. Associations with insects, plants, and animals can therefore influence how these bacteria persist and spread. Examining those relationships helps researchers investigate host-microbe interactions while also clarifying how bacterial life strategies vary across different biological environments.
Spiroplasma research connects unusual cellular structure with broader questions about bacterial evolution. Its wall-less organization, flexible morphology, and reliance on host-associated resources provide a context for examining how bacteria adapt to distinct lifestyles. The genus also links evolutionary studies with insect biology, plant disease, and host-microbe interactions, allowing researchers to compare related biological pressures across systems.
Certain Spiroplasma species serve as models for studying reproductive manipulation and biological control. These research uses make the genus valuable beyond basic bacterial characterization, because they examine how an associated microorganism may influence host biology or support strategies for managing biological populations. The overview identifies these applications as research directions rather than as properties shared by every species.
Spiroplasma is relevant to plant disease research because some members of the genus form associations involving plants, while broader studies examine strategies for managing agricultural pathogens. This work connects bacterial biology with practical agricultural concerns. Researchers can use the genus to investigate host-associated disease processes and to develop approaches intended to limit pathogen-related effects in crops.