Srs Microscopy
Srs Microscopy - Related Videos
11:16
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Cited by 12 •
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A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 105 •
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This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
09:20
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
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Cited by 16 •
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This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.
12:28
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
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Cited by 11 •
2017
This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.
FAQs
Contrast depends on the frequency difference between the synchronized pump and Stokes beams. When that difference matches a molecular vibration, the sample reaches vibrational resonance and produces stimulated Raman gain or loss. The resulting interaction changes the transmitted light intensity, allowing the microscope to distinguish regions containing molecules with the targeted vibrational signature.
Stimulated Raman gain or loss provides the measurable signal used to construct an image. Rather than relying on a fluorescent label, SRS microscopy detects a rapid change in transmitted intensity caused by molecular resonance. Spatially recording that change converts local chemical differences into an image of molecular composition across cells, tissues, or other biological samples.
Each targeted molecular vibration supplies a chemical signature that guides image contrast. By tuning the frequency relationship between the pump and Stokes beams to that signature, SRS microscopy can selectively map constituents such as lipids, proteins, or metabolites. This mechanism connects the measured optical response with the distribution of specific biological components.
SRS microscopy obtains molecular contrast without fluorescent labels, whereas fluorescence-based imaging generally depends on labeled targets for visualization. Its label-free operation is especially relevant when researchers need to examine living cells or tissues while preserving their native molecular constituents. The technique therefore combines chemical specificity with compatibility for observing biological organization and processes in live specimens.
The measurement begins with synchronized pump and Stokes laser beams directed toward the biological sample. Their frequency difference is selected to correspond to a molecular vibration of interest. At resonance, the system records the stimulated Raman gain or loss as a change in transmitted light intensity, and spatially collected signals generate a chemically informative image.
SRS microscopy can map several classes of constituents, including lipids, proteins, and metabolites, along with other molecular components that possess relevant vibrational signatures. This range allows investigators to examine chemical composition rather than only structural appearance. In biological specimens, the resulting maps can reveal how different constituents are distributed within cells and tissues.
The method is useful for studying cell organization, metabolism, disease processes, and tissue structure. Its combination of rapid imaging, molecular contrast, and label-free operation supports work with living cells and tissues. Researchers can therefore investigate chemical and spatial changes while retaining specimens in conditions compatible with observing biological structure and activity.