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JoVE Journal
Developmental Biology

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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
 

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Article DOI: 10.3791/56113 12:28 min November 10th, 2017
November 10th, 2017

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Summary

This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.

Transcript

Tags

Correlative Super-resolution Electron Microscopy Protein Localization Zebrafish Retina Microscopy Method Subcellular Organelles Larval Zebrafish Retinal Development Cellular Pathway Protein Mislocalization Mutants Technique Structural Context Tokuyasu Sections Silicone Wafers Platinum Shadowing Topography Larva Zebrafish Retina Studies Biological Systems Protein Expression Mouse Tissue
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