Stem Imaging

Stem imaging is the visualization and analysis of biological stems or stem cells to reveal their structure, behavior, and organization. In biology, imaging methods use light, fluorescence, or other contrast mechanisms to distinguish cellular components and capture changes through microscopy, including time-lapse observations of growth, division, migration, or differentiation. These approaches help researchers examine tissue development, stem cell maintenance, regeneration, and interactions with surrounding cells. Quantitative image analysis can connect visible patterns with molecular and cellular processes, supporting studies of development, disease, tissue engineering, and potential regenerative therapies.

Stem Imaging - Related Videos

Research

JoVE Journal - Neuroscience

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

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Cited by 11 •

2012

This article describes an optimized sequence of events for multimodal imaging of cellular grafts in rodent brain using: (i) in vivo bioluminescence and magnetic resonance imaging, and (ii) post mortem histological analysis. Combining these imaging modalities on a single animal allows cellular graft evaluation with high resolution, sensitivity and specificity.

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

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Cited by 33 •

2008

This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

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Cited by 15 •

2014

Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.

Research

JoVE Journal - Medicine
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Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

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Cited by 26 •

2011

A compartmentalizing microfluidic device for investigating cancer stem cell migration is described. This novel platform creates a viable cellular microenvironment and enables microscopic visualization of live cell locomotion. Highly motile cancer cells are isolated to study molecular mechanisms of aggressive infiltration, potentially leading to more effective future therapies.

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

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Cited by 8 •

2007

This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.

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