Calu-3 Cell Culture
Calu-3 Cell Culture - Related Videos
12:09
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
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Cited by 25 •
2013
The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
0 Views •
Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
09:06
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
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Cited by 7 •
2019
Using human primary prostate epithelial cells, we report a novel biomarker-free method of functional characterization of stem-like cells by a spheroid-based label-retention assay. A step-by-step protocol is described for BrdU, CFSE, or Far Red 2D cell labeling; three-dimensional spheroid formation; label-retaining stem-like cell identification by immunocytochemistry; and isolation by FACS.
04:02
A Hyaluronic Acid-Based Hydrogel for 3-Dimensional Culture of Glioblastoma Cells
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2025
This video demonstrates how gliomaspheres, derived from brain tumors, are separated into single cells and encapsulated in a hyaluronic acid-based hydrogel. This process promotes cell-cell interactions, forming a structured, three-dimensional glioblastoma model.
04:45
3-Dimensional Culture of Lung Carcinoma Cells: A Method To Study Cell-Matrix Interactions
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2023
This video describes the technique of 3-D culture of lung carcinoma cells to study the cell-matrix interactions. In the protocol, we will perform the 3D culture of TUM622 lung carcinoma cells to explore its potential of forming organoids in vitro.
FAQs
Permeable supports allow Calu-3 cells to form layers with distinct surfaces, supporting polarization and the development of tight junctions. This organization is important because it creates a more tissue-like arrangement than an undifferentiated cell layer. Researchers can therefore examine how airway epithelial structure relates to barrier integrity, epithelial transport, and responses to compounds or infectious agents.
Air-liquid interface conditions help Calu-3 cultures develop features that resemble the airway surface. Exposing the relevant surface to air provides a model for studying surface-associated airway processes, including mucus-related behavior and interactions with inhaled substances. This configuration is especially useful when researchers want experimental conditions that more closely reflect the biology of respiratory epithelium.
Polarization separates the epithelial layer into organized surface domains, allowing researchers to study directional transport and barrier behavior. In Calu-3 cultures, this organization connects cellular structure with functions relevant to airway biology. It also provides a basis for evaluating whether a treatment, exposure, or disease-related stimulus alters epithelial responses rather than merely affecting cells in an unstructured layer.
A basic workflow places Calu-3 cells on a permeable support and allows them to develop an organized epithelial layer. Researchers may then maintain the culture under air-liquid interface conditions when airway-surface features are needed. The resulting model can be examined for barrier integrity, transport behavior, mucus-related processes, or responses to experimental exposures, depending on the study objective.
Calu-3 cultures are useful when an inhaled drug study requires an airway-relevant epithelial barrier rather than a generic cell layer. Their polarized organization supports investigation of epithelial transport and interactions between the respiratory surface and therapeutic substances. This makes the model relevant for evaluating delivery strategies and examining how candidate treatments behave at a cultured airway interface.
Researchers can use Calu-3 cultures to investigate disease-related epithelial responses and interactions between respiratory tissues and infectious agents. The model provides a controlled in vitro setting for examining changes in barrier integrity and cellular behavior during these challenges. It can also support assessment of therapeutic strategies by linking treatment effects to airway epithelial structure and function.