Strand-displacing Polymerase
Strand-displacing Polymerase - Related Videos
03:02
Microsphere Polymerase Chain Reaction to Amplify Single-Stranded DNA
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2025
This video describes the technique of amplifying single-stranded DNA using oligonucleotide-coated polymer microspheres in a single-tube reaction. This process yields pure single-stranded DNA, which can be further used for DNA sequencing and microarray analysis.
07:50
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Cited by 6 •
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This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.
11:05
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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We describe a simple lithographic procedure for the immobilization of gene-length DNA molecules on a surface, which can be used to perform cell-free gene expression experiments on biochips.
02:10
Translesion DNA Polymerases
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2020
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication. TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
13:27
PCR: The Polymerase Chain Reaction
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2023
The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling – cyles of temperature changes at fixed time intervals. Using a thermostable DNA polymerase, PCR can create numerous copies of DNA from DNA building blocks called dNTPs. There are three steps in PCR: denaturation, annealing, and elongation. Denaturation is the first step in the cycle and causes the DNA to melt by disrupting hydrogen bonds between the bases resulting in single-stranded DNA. Annealing...
FAQs
As the primer is extended, the newly synthesized strand encounters a downstream region that remains complementary to the template. Strand-displacing activity allows the growing strand to push this paired region aside rather than requiring its prior separation by heat. This combination of primer extension and displacement keeps synthesis moving through previously paired DNA and supports continuous amplification under constant-temperature conditions.
Its displacement activity removes the need to repeatedly separate paired DNA by high-temperature treatment. Because synthesis can continue while the enzyme separates the downstream complementary strand, amplification methods can operate at a constant temperature. This simplifies the temperature-control requirements and reduces reliance on thermal cycling equipment, which is particularly relevant for rapid or field-based nucleic acid detection.
Strong processivity allows the enzyme to remain productive during extension and synthesize DNA efficiently over the template. In combination with strand displacement, this supports sustained production of amplified material rather than requiring frequent interruptions for template separation. The resulting capability is important when applications demand rapid detection, whole-genome amplification from limited samples, or analysis of specific mutations.
The enzyme supports several isothermal amplification formats, including rolling-circle amplification, loop-mediated amplification, and multiple-displacement amplification. These methods share the practical advantage of avoiding repeated high-temperature denaturation, although the overview does not assign a separate mechanism or application to each format. Together, they extend the enzyme's use across nucleic acid detection, limited-sample amplification, and mutation analysis.
A general workflow begins with a primer positioned for DNA synthesis, followed by extension under a constant-temperature condition. As synthesis proceeds, the enzyme displaces a downstream complementary strand, allowing amplification to continue without repeated thermal denaturation. The resulting nucleic acid amplification can then support detection, whole-genome amplification, or mutation analysis, depending on the selected assay format.
Researchers may select it when they need rapid nucleic acid detection, amplification from a limited sample, whole-genome amplification, or mutation analysis without depending heavily on thermal cycling equipment. Its constant-temperature operation also suits field-based diagnostics, where reducing equipment requirements can be valuable. These applications connect the enzyme's molecular mechanism with practical needs in biology and biotechnology.