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JoVE Journal
Biology

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
 

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Article DOI: 10.3791/53087
November 25th, 2015

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Summary November 25th, 2015

This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

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