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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Article DOI: 10.3791/53087 • 07:50 min • November 25th, 2015
November 25th, 2015 •
1, 1, 1,2
1Department of Electrical Engineering, University of Washington, 2Department of Computer Science & Engineering, University of Washington

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This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

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Plasmid-derived DNA DNA Strand Displacement Gates Chemical Reaction Networks DNA Nanotechnology Plasmid DNA Bacterial Culture Bacterial Glycerol Stocks Double-stranded DNA DNA Nanostructures NdsDNA Gates Enzymatic Processing Steps Fluorescence Kinetics Measurements Arbitrary Chemical Reaction Networks (CRNs) Prescriptive Molecular Programming Language Multi-step Reaction Cascade Catalytic Kinetics
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