Stratum Corneum
Stratum Corneum - Related Videos
08:00
Measuring and Modeling Contractile Drying in Human Stratum Corneum
0 Views •
Cited by 2 •
2017
This article describes a method of quantifying the dynamic drying behavior and mechanical properties of stratum corneum by measuring spatially resolved in-plane drying displacements of circular tissue samples adhered to an elastomer substrate. This technique can be used to measure how different chemical treatments alter drying and tissue mechanical properties.
09:32
Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
0 Views •
Cited by 1 •
2019
Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.
05:37
Visualization of Mitochondria within Intraepidermal Nerve Fibers
0 Views •
2025
Source: Hamid, H. S., et al. Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers. J. Vis. Exp. (2017) This video demonstrates the method for visualizing nerve-specific mitochondria using three-dimensional imaging. It outlines the process of acquiring sequential scans of pre-labeled intraepidermal nerve fibers and generating 3D surface images to isolate and display mitochondria in relation to nerve structures.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
0 Views •
2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
08:26
Dissolving Microneedle Array Patches Manufactured By Solvent Casting Technique and Essential Characterization of Microneedle-Based Biomedical Devices
0 Views •
2026
The present work aims to serve as a practical guide enabling researchers to prepare their own DMAP devices using the solvent-casting method, also known as micromolding, and to perform basic characterization.
FAQs
Corneocytes and the surrounding lipids contribute different parts of the permeability barrier. The flattened cells create a densely organized cellular component, while the lipid-rich matrix fills the spaces between them. This arrangement restricts passage rather than creating an absolutely impermeable surface, allowing researchers to examine how barrier organization influences water loss, chemical exposure, and microbial protection.
Controlled desquamation, or shedding of the outer cells, is an important counterpart to barrier formation. The stratum corneum must limit permeability while permitting this controlled removal of surface material. In biological studies, examining desquamation alongside corneocyte and lipid organization helps place the skin surface in context when investigating barrier dysfunction and dermatological disorders.
Composition alone does not fully describe barrier behavior; the spatial organization of corneocytes and lipids is also relevant. Because this structure helps limit permeability, researchers use composition and organization when studying skin hydration and barrier dysfunction. This perspective connects the arrangement of barrier components with broader questions about the condition and performance of the skin barrier.
The stratum corneum offers a specific biological context for studying inflammation because barrier dysfunction and inflammation are examined together in skin research. Its composition and organization help researchers assess dermatological disorders, while barrier performance provides a way to frame investigations involving chemical exposure, water loss, and environmental microbes. This makes it relevant to both basic biology and biomedical research.
Studies of topical formulations use the stratum corneum as a key reference point because its composition and organization influence permeability. Researchers can evaluate formulations in relation to the barrier’s ability to restrict passage, while also considering skin hydration and barrier dysfunction. These investigations support assessment of dermatological products and the development of approaches intended to improve transport across skin.
Percutaneous drug delivery research focuses on how therapeutic compounds cross skin despite the stratum corneum’s permeability-limiting organization. Understanding this barrier helps researchers evaluate transport and develop strategies for improving delivery. The topic therefore connects basic biology, including corneocyte and lipid arrangement, with biomedical efforts to improve the movement of therapeutic compounds across skin.