Surface Antigen Profiling
Surface Antigen Profiling - Related Videos
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
13:05
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Cited by 39 •
2014
We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.
14:11
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Cited by 21 •
2016
We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
05:04
Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen
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2025
In this video, we demonstrate the process for detecting antibody isotypes in multiple human serum samples using a microarray slide printed with antigenic subtypes of influenza virus strains.
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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Cited by 9 •
2016
In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).
FAQs
Antigen-specific antibodies bind selected molecules exposed on the cell membrane, while attached fluorescent labels make that binding detectable. Measuring the resulting signals across labeled cells allows researchers to determine which markers are present and compare their expression patterns. The selected antibody panel therefore determines which aspects of cell identity, state, or function the analysis can resolve.
A single marker may be shared by multiple cell populations, whereas a pattern across several antigens can distinguish them more effectively. Comparing combinations helps reveal cellular heterogeneity and supports classification of immune, stem, cancer, or developing cells. This multiparameter view can also identify subpopulations whose differences would be missed when each marker is considered alone.
Researchers compare marker patterns across samples to identify shifts in the presence or expression of selected antigens. Differences may indicate altered cell composition, differentiation, disease-associated states, or responses to treatment. Interpreting these changes requires examining the overall profile rather than relying on one signal, because related populations can differ across several markers simultaneously.
In biology, the method connects externally displayed molecules with broader questions about what cells are and how they behave. Profiling supports immune-cell classification, stem and cancer cell characterization, and developmental research. It can also help monitor cellular responses to disease or treatment, making marker patterns useful for tracking state changes over time or between conditions.
A typical workflow selects antibodies against the surface antigens of interest, couples or uses them with fluorescent labels, applies them to the cells, and measures antibody binding with flow cytometry or a related immunodetection method. The resulting marker measurements are then compared across cells or samples to classify populations and identify differences in antigen patterns.
The essential detection components are cells, antigen-specific antibodies, and fluorescent labels that make bound antibodies measurable. Flow cytometry is a commonly used analytical method, while related immunodetection approaches may also be applied. Together, these components provide measurements of selected surface markers, allowing investigators to examine cell populations according to their molecular profiles.
Researchers can apply surface antigen profiling when they need to monitor how cell populations change during disease or after treatment. Comparing profiles between conditions may reveal altered cellular states, heterogeneous responses, or candidate biomarkers. The findings can contribute to diagnostic or prognostic investigations and may highlight surface molecules suitable for therapeutic targeting.
Comparative profiles can identify distinct cell populations, reveal heterogeneity, and track differentiation during development or experimental treatment. They may also expose marker patterns associated with disease, prognosis, or potential therapeutic targets. The value lies in linking measurable surface changes across samples with changes in cellular identity, state, or response.