Synchrotron Data Collection
Synchrotron Data Collection - Related Videos
01:29
Data Collection II
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2023
The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and family,...
01:30
Data Collection I
0 Views •
2023
Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of data...
08:51
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Cited by 9 •
2008
We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.
01:13
Data Collection by Experiments
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2023
Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.” An example of the experimental method is a public clinical trial...
01:07
Data Collection by Survey
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2023
The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
FAQs
Crystal rotation exposes the protein or nucleic acid crystal to the synchrotron beam while diffraction is recorded. The resulting patterns provide data for computational interpretation. This workflow converts X-ray measurements into electron-density information, which can then support construction of a three-dimensional molecular model for examining the organization of the biological molecule.
The beam’s intensity and tight focus are central to the method’s performance. They allow biological crystals to be measured with exceptional speed and precision, producing diffraction data suitable for structural interpretation. These characteristics make synchrotron collection valuable when researchers need detailed molecular information about biomolecular organization, ligand interactions, or drug-related structural questions.
Computational interpretation links recorded diffraction patterns to electron density, a map used to determine how molecular material is organized within the crystal. From that information, researchers obtain a three-dimensional view of a protein or nucleic acid. The resulting model can reveal how biomolecules interact with ligands or drugs, supporting studies of molecular mechanisms.
In macromolecular crystallography, the approach is suited to crystals of proteins and nucleic acids. The resulting structural information applies to enzymes, receptors, and other biomolecules, allowing researchers to examine their organization and interactions with ligands or drugs. This connects crystallographic measurement with mechanism-based questions in biology and structural research.
A typical workflow begins with a protein or nucleic acid crystal, places it in the focused synchrotron X-ray beam, and records diffraction while the crystal rotates. Computational analysis then interprets the diffraction patterns to generate electron-density information and determine a three-dimensional molecular structure. The model can subsequently be examined for ligand or drug interactions.
Researchers choose it when structural biology projects require rapid, precise measurements of biological crystals and detailed molecular models. The resulting structures support mechanism-based research, protein engineering, and therapeutic development. Because the data can show how enzymes, receptors, and other biomolecules interact with ligands or drugs, the approach also supports drug-related structural investigations.
Structural models from these measurements show how a biomolecule is organized and how it interacts with a ligand or drug. For enzymes and receptors, that information supports studies of molecular mechanisms and provides a structural basis for protein engineering or therapeutic development. The outcome is a detailed connection between measured diffraction data and biological research questions.