Syringe Infiltration
Syringe Infiltration - Related Videos
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
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A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
14:24
Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration
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Three-dimensional (3D) microstructured composite beams are fabricated through the directed and localized infiltration of nanocomposites into 3D porous microfluidic networks. The flexibility of this manufacturing method enables the utilization of different thermosetting materials and nanofillers in order to achieve a variety of functional 3D reinforced nanocomposite macroscopic products.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
02:12
Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing
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Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
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This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
FAQs
Gentle pressure from the syringe drives the aqueous suspension through stomata on the leaf surface. Once inside, the liquid occupies the apoplast, the extracellular space between plant cells. This route makes internal tissue accessible while keeping delivery localized, which supports direct observation or later sampling of the treated area.
The needleless syringe is pressed against the underside because the suspension enters through stomata located on the leaf surface. Applying pressure at this contact area helps move the liquid into the apoplast rather than leaving it on the exterior. The resulting infiltrated region can then be distinguished from untreated tissue.
A water-soaked appearance marks the portion of tissue reached by the introduced suspension. This visible change helps researchers identify the localized treatment area for monitoring or sampling. Because the infiltrated region can be separated from surrounding tissue visually, observations can focus on responses associated with the delivered stain, chemical treatment, microbe-related experiment, or other suspension.
Researchers place an aqueous suspension against the underside of a leaf using a needleless syringe, then apply gentle pressure to move the liquid through the stomata. The treated region becomes water-soaked, providing a visual indication of infiltration. That area may subsequently be monitored or sampled, depending on the biological experiment.
Syringe Infiltration supports transient gene expression experiments and the delivery of staining solutions into plant tissue. It can also introduce treatments used to analyze plant responses to microbes or chemical compounds. These applications take advantage of localized delivery, allowing researchers to examine a defined leaf region rather than treating the entire plant.
The technique offers a simple approach for rapid, localized experiments without specialized infiltration equipment. A syringe can deliver the suspension directly to a selected leaf area, while the water-soaked region identifies where treatment occurred. This combination of accessible equipment and spatially limited delivery is useful for screening treatments or monitoring plant responses.