Tissue Processing
Tissue Processing - Related Videos
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
13:08
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Cited by 80 •
2013
A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.
02:41
Cryopreserved Tissue Section Fixation and Staining: A Procedure to Process Frozen Brain Tissue Sections for Laser Microdissection
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2023
This video presents the fixation and staining method to process frozen mouse brain tumor tissue. The processed tissue helps differentiate and obtain a specific population of cells during subsequent laser capture microdissection.
06:56
Wet-spinning-based Molding Process of Gelatin for Tissue Regeneration
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Cited by 5 •
2019
We developed and describe a protocol based on the wet spinning concept, for the construction of gelatin-based biomaterials used for the application of tissue engineering.
03:16
Processing of Infected Tissues for Bacterial Enumeration in a Mouse Co-Infection Model
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2026
Source: Lenhard, A., et al. A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness. J. Vis. Exp. (2022)This video demonstrates the processing of lungs from a mouse co-infected with a pathogenic bacterium and a virus. Mincing, homogenizing, and plating the lung tissue allows quantification of bacterial load, indicating bacterial spread to the lungs and disease severity.
FAQs
Fixation stabilizes tissue before later handling, so cellular structures remain suitable for examination as water is removed and the specimen is prepared for embedding. Performing dehydration first would disrupt the intended sequence because the tissue would not yet have undergone the stabilizing step described for this workflow. This ordering supports preservation during subsequent sectioning and staining.
Graded alcohols remove water progressively, while clearing with an organic solvent prepares the dehydrated specimen for infiltration by paraffin wax. These stages are complementary rather than interchangeable: dehydration changes the tissue’s solvent environment, and clearing bridges that environment with the supporting medium. Together, they allow the specimen to become embedded for thin sections used in microscopy.
Paraffin infiltration provides a supporting medium around the processed tissue, and embedding creates a block that can be cut into thin slices. Sectioning matters because microscopy requires a sufficiently thin specimen for cellular structures to be examined. The transition from infiltration to sectioning therefore determines whether tissue organization can be displayed clearly after staining.
Staining reveals specific structures that might not be readily distinguished in an unstained section. It converts the thin slice into a more informative microscopic preparation, allowing investigators to examine cellular organization within preserved tissue. In biology and medicine, this added visibility supports interpretation of tissue architecture, disease-related changes, and cellular function.
The workflow supports several fields because it produces tissue preparations suitable for microscopic study. Histology uses these preparations to examine tissue organization, pathology uses them to investigate disease-related changes, and developmental biology uses them to study tissues during development. The same general sequence therefore connects structural examination with broader biological and medical questions.
Microscopic examination of stained, processed sections can show how cells are arranged within tissues and can reveal changes associated with disease. These preparations also support investigation of cellular function by placing individual cellular structures in their tissue context. As a result, researchers can relate microscopic patterns to tissue organization, developmental processes, and biological or medical conditions.