Transgenic Fish
Transgenic Fish - Related Videos
07:45
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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Cited by 4 •
2013
In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
05:12
Swimming Performance Assessment in Fishes
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Cited by 56 •
2011
The lives of the majority of fish are predicated on swimming. This protocol describes techniques for capturing a range of swimming modes available to individual and schooling fish, and includes metrics associated with swimming physiology and behaviour.
02:16
Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
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2025
This video demonstrates the generation of neural progenitor cells from non-transgenic and transgenic human pluripotent stem cells (hPSCs). hPSCs are differentiated into neural progenitor cells using a neuronal differentiation medium. While differentiation is induced in non-transgenic cells using small molecules, the transgenic hPSCs are differentiated by adding an antibiotic that induces the promoter for a neuronal differentiation-specific transcription factor.
FAQs
A regulatory promoter controls when and where an introduced gene is expressed. This control links the inserted DNA sequence to a measurable biological activity or trait, rather than merely placing foreign DNA in the genome. Researchers can therefore investigate how gene regulation influences development, physiology, growth, metabolism, or responses to environmental conditions.
Integration into the developing genome allows researchers to examine the behavior of an introduced sequence within a living organism. Its expression can then be related to biological traits, including growth, metabolism, development, or environmental responses. Studying these outcomes also helps researchers evaluate how genetic changes and associated traits are inherited.
Fluorescent reporter genes provide a visible signal that reveals biological activity in the fish. When expression occurs under suitable regulatory control, fluorescence can help researchers observe where or when the introduced genetic program is active. This makes gene function and developmental processes easier to track than measurements based only on an unseen DNA change.
Researchers begin by preparing recombinant DNA that contains the selected gene or modified DNA sequence and an appropriate regulatory promoter. They introduce this construct into fertilized fish eggs, where it may integrate into the genome during development. The resulting fish can then be examined for gene expression, biological traits, and inheritance-related outcomes.
These fish allow researchers to connect altered genetic information with observable biological outcomes. Applications described for the system include investigating gene function, development, physiology, disease mechanisms, and responses to environmental conditions. Because reporter genes or trait-affecting genes can produce measurable changes, the animals provide models for studying regulation in an intact organism.
Genes affecting growth or metabolism can help researchers evaluate whether genetic modification produces potentially useful traits for aquaculture or biotechnology. At the same time, examining trait inheritance and biological effects supports assessment of possible benefits and risks. This combined approach connects experimental findings with decisions about how modified fish might be developed or evaluated.