Transport Stoichiometry

Transport stoichiometry is the quantitative relationship between the numbers of ions or molecules a membrane transporter moves during each transport cycle, a property that determines how cells control solute distribution and energy use. Transporters achieve this ratio through specific binding sites and conformational changes, coupling the movement of one substrate to one or more ions, such as sodium or hydrogen, down an electrochemical gradient; some systems use ATP-driven pumping to establish those gradients. In biology, stoichiometry helps explain membrane potential, nutrient uptake, pH regulation, and secondary active transport. Measuring it also clarifies transporter function and supports models of cellular metabolism and physiological homeostasis.

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JoVE Core - Chemistry

Reaction Stoichiometry

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2020

A balanced chemical equation provides a great deal of information in a very succinct format. Chemical formulas provide the identities of the reactants and products involved in the chemical change, allowing classification of the reaction. Coefficients provide the relative numbers of these chemical species, allowing a quantitative assessment of the relationships between the amounts of substances consumed and produced by the reaction. These quantitative relationships are known as the reaction’s...

Stoichiometry, Product Yield, and Limiting Reactants - Concepts

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2020

Chemical equations represent how a chemical reaction proceeds from reactants to products through physical or chemical change using chemical formulas. Stoichiometry is a term that describes the relative quantities of reactants and products in a chemical reaction. It is based on the Law of Conservation of Mass, which is a fundamental law that states that matter is neither created nor destroyed. Quite simply, the number and identity of reactant atoms must equal the number and identity of product...

Stoichiometry, Product Yield, and Limiting Reactants - Student Protocol

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Synthesis of tris(ethylenediamine)nickel chlorideExpand In this lab, you will combine nickel chloride hexahydrate and ethylenediamine in water to produce tris(ethylenediamine)nickel chloride. When nickel chloride hexahydrate dissolves in water, it rearranges to become the hexaaquanickel cation and two chloride anions. Adding ethylenediamine results in three ethylenediamine molecules exchanging with the six water molecules. This...

Stoichiometry, Product Yield, and Limiting Reactants - Instructor Prep

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of 25% v/v Ethylenediamine in Deionized WaterExpand Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Note: Ethylenediamine is toxic, caustic, flammable, and corrosive. This solution must be...

Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles

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2020

Chemical stoichiometry describes the quantitative relationships between reactants and products in chemical reactions. In addition to measuring quantities of reactants and products using masses for solids and volumes in conjunction with the molarity for solutions; now, the gas volumes can also be used to indicate quantities. If the volume, pressure, and temperature of a gas is known, then the ideal gas equation to calculate how many moles of the gas are present, can be used. Conversely, if the...

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