Vascular Integrity
Vascular Integrity - Related Videos
08:50
Assessment of Vascular Function in Patients With Chronic Kidney Disease
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Cited by 25 •
2014
The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.
08:18
Engineered Vascularized Muscle Flap
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Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
12:38
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
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Cited by 12 •
2017
Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.
11:35
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
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Cited by 16 •
2010
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
FAQs
Endothelial junctions help determine how readily substances pass between neighboring cells. Tight junctions and adherens junctions work within the endothelial layer, while the basement membrane, pericytes, and extracellular matrix provide supporting control of vessel stability. Because these elements interact rather than act independently, changes in junction organization can alter barrier behavior without requiring loss of the entire vessel structure.
Mechanical and chemical signals help coordinate how vessel-supporting components respond to changing conditions. Their effects can influence permeability and vessel stability through interactions among endothelial cells, junctions, pericytes, the basement membrane, and extracellular matrix. Studying these responses is important because vessels must adjust their barrier behavior while maintaining organized tissue support.
These biological conditions can alter the endothelial barrier and disturb the coordinated structures that normally support vessel function. The resulting changes may appear as increased permeability, altered junction organization, or modified tissue perfusion. Comparing these responses across inflammation, infection, injury, and tumors helps investigators distinguish how different pathological settings affect vascular behavior.
Three major readouts are changes in permeability, junction organization, and tissue perfusion. Permeability indicates altered barrier behavior, junction organization shows structural changes among endothelial cells, and perfusion reflects how effectively blood moves through tissue. Using these measures together provides a broader assessment than relying on a single indicator of vessel function.
A study can focus on one or more complementary outcomes: permeability, endothelial junction organization, or tissue perfusion. Investigators can then examine how these outcomes change in the context of inflammation, infection, injury, or tumors. This approach connects measurable vascular changes with the biological condition being studied while also supporting comparisons among different experimental contexts.
Vascular integrity research provides a framework for investigating edema, angiogenesis, and cardiovascular disease, all of which involve changes in vessel behavior or support. It also informs studies of therapies intended to preserve or restore vessel function. By tracking permeability, junction organization, or perfusion, researchers can evaluate whether vascular responses remain stable or improve.